Tamarin O, Comeau S, Déjous C, Moynet D, Rebière D, Bezian J, Pistré J
IXL (CNRS UMR 5818-ENSEIRB-Université Bordeaux1), 351 Cours de la Libération, 33045, Talence, France.
Biosens Bioelectron. 2003 May;18(5-6):755-63. doi: 10.1016/s0956-5663(03)00022-8.
Love wave sensors (ST-cut quartz substrate with interdigital transducers, SiO(2) guiding layer and sensitive coating) have been receiving a great deal of attention for a few years. Indeed, the wave coupled in a guiding layer confers a high gravimetric sensitivity and the shear horizontal (SH) polarization allows to work in liquid media. In this paper, an analytical method is proposed to calculate the Love wave phase velocity and the gravimetric sensitivity for a complete multilayer structure. This allows us to optimize the Love wave devices design in order to improve their gravimetric sensitivity in liquid media. As a model for virus or bacteria detection in liquids (drinking or bathing water, food em leader ) we design a model using M13 bacteriophage. The first step is the anti-M13 (AM13) monoclonal antibody grafting, on the device surface (SiO(2)). The second step is an immunoreaction in between the M13 bacteriophage and the AM13 antibody. The Love wave device allows to detect in real time the graft of the AM13 sensitive coating, as well as the immobilization of the M13 bacteriophages. With a pH change, the M13 bacteriophages can be removed from the sensor surface, in order to be numerated as plaque forming unit (pfu). Results on the sensitivity of Love waves are compared with similar immunological works with bulk acoustic wave devices, and demonstrate the high potentialities of Love waves sensors.
多年来，洛夫波传感器（具有叉指换能器、SiO₂ 波导层和敏感涂层的 ST 切石英基底）一直备受关注。实际上，在波导层中耦合的波具有很高的重量灵敏度，并且水平剪切（SH）极化使得该传感器能够在液体介质中工作。本文提出了一种分析方法，用于计算完整多层结构的洛夫波相速度和重量灵敏度。这使我们能够优化洛夫波器件的设计，以提高其在液体介质中的重量灵敏度。作为液体（饮用水、洗澡水、食品等）中病毒或细菌检测的模型，我们设计了一个使用 M13 噬菌体的模型。第一步是在器件表面（SiO₂）上接枝抗 M13（AM13）单克隆抗体。第二步是 M13 噬菌体与 AM13 抗体之间的免疫反应。洛夫波器件能够实时检测 AM13 敏感涂层的接枝以及 M13 噬菌体的固定。随着 pH 值的变化，可以将 M13 噬菌体从传感器表面去除，以便将其计数为噬菌斑形成单位（pfu）。将洛夫波的灵敏度结果与使用体声波器件的类似免疫学研究进行了比较，证明了洛夫波传感器具有很高的潜力。
