Rein D T, Roehrig K, Schöndorf T, Lazar A, Fleisch M, Niederacher D, Bender H G, Dall P
Department of Gynecology and Obstetrics, University of Düsseldorf, Germany.
J Cancer Res Clin Oncol. 2003 Mar;129(3):161-4. doi: 10.1007/s00432-003-0415-0. Epub 2003 Feb 20.
Interactions of hyaluronic acid (HA) with its binding protein RHAMM (receptor for HA-mediated motility) have been proposed as being important in promoting tumour progression and dissemination. This comparative study was designed to investigate the RHAMM expression patterns in endometrial carcinoma.
We examined a series of 89 endometrial carcinomas and 15 normal endometrial tissues by immunohistochemistry, using a RHAMM-specific polyclonal antibody. Expression of RHAMM was assessed according to the pattern and intensity within (overall cytoplasm, center/periphery of tumours) and between the tumours. The staining results were compared to the corresponding clinical data (age, menopause status, histological staining, histological grading, lymph node status).
RHAMM-expression was detectable in 58% of the 89 tumours [Histological stage: pT1a (8/12); pT1b (16/37); pT1c (18/26); pT2 (6/9); pT3a (4/5)] and 13% (2/15) of the normal endometrial tissues. The positivity rates for RHAMM were 100% in patients with positive lymph nodes but only 50.7% in patients with negative lymph nodes ( P<0-01). Additionally, the expression pattern showed a highly significant correlation ( P<0.01) with the histological grade of the tumours [G1 (6/42), G2 (33/34), G3 (13/13)] and occurrence of lymph node metastases.
Our results suggest that RHAMM expression may enhance and improve the invasion and metastasis of endometrial carcinomas.
有人提出透明质酸（HA）与其结合蛋白RHAMM（HA介导的运动受体）之间的相互作用在促进肿瘤进展和扩散方面具有重要意义。本比较研究旨在调查子宫内膜癌中RHAMM的表达模式。
我们使用RHAMM特异性多克隆抗体，通过免疫组织化学检查了89例子宫内膜癌和15例正常子宫内膜组织。根据肿瘤内部（整个细胞质、肿瘤中心/周边）以及肿瘤之间的模式和强度评估RHAMM的表达。将染色结果与相应的临床数据（年龄、绝经状态、组织学染色、组织学分级、淋巴结状态）进行比较。
在89例肿瘤中，58%可检测到RHAMM表达[组织学分期：pT1a（8/12）；pT1b（16/37）；pT1c（18/26）；pT2（6/9）；pT3a（4/5）]，而在正常子宫内膜组织中为13%（2/15）。淋巴结阳性患者的RHAMM阳性率为100%，而淋巴结阴性患者仅为50.7%（P<0.01）。此外，表达模式与肿瘤的组织学分级[G1（6/42），G2（33/34），G3（13/13）]和淋巴结转移的发生高度相关（P<0.01）。
我们的结果表明，RHAMM表达可能增强和促进子宫内膜癌的侵袭和转移。
