Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, No. 17 Yongwaizheng Street, Nanchang, 330006, Jiangxi, China.
Huankui Academy, Nanchang University, Honggutan New District, Jiangxi, 330006, Nanchang, China.
BMC Cancer. 2023 Feb 7;23(1):125. doi: 10.1186/s12885-023-10596-w.
Gliomas are the most frequent type of central nervous system tumor, accounting for more than 70% of all malignant CNS tumors. Recent research suggests that the hyaluronan-mediated motility receptor (HMMR) could be a novel potential tumor prognostic marker. Furthermore, mounting data has highlighted the important role of ceRNA regulatory networks in a variety of human malignancies. The complexity and behavioural characteristics of HMMR and the ceRNA network in gliomas, on the other hand, remained unknown.
Transcriptomic expression data were collected from TCGA, GTEx, GEO, and CGGA database.The relationship between clinical variables and HMMR was analyzed with the univariate and multivariate Cox regression. Kaplan-Meier method was used to assess OS. TCGA data are analyzed and processed, and the correlation results obtained were used to perform GO, GSEA, and ssGSEA. Potentially interacting miRNAs and lncRNAs were predicted by miRWalk and StarBase.
HMMR was substantially expressed in gliomas tissues compared to normal tissues. Multivariate analysis revealed that high HMMR expression was an independent predictive predictor of OS in TCGA and CGGA. Functional enrichment analysis found that HMMR expression was associated with nuclear division and cell cycle. Base on ssGSEA analysis, The levels of HMMR expression in various types of immune cells differed significantly. Bioinformatics investigation revealed the HEELPAR-hsa-let-7i-5p-RRM2 ceRNA network, which was linked to gliomas prognosis. And through multiple analysis, the good predictive performance of HELLPAR/RRM2 axis for gliomas patients was confirmed.
This study provides multi-layered and multifaceted evidence for the importance of HMMR and establishes a HMMR-related ceRNA (HEELPAR-hsa-let-7i-5p-RRM2) overexpressed network related to the prognosis of gliomas.
神经胶质瘤是最常见的中枢神经系统肿瘤，占所有恶性中枢神经系统肿瘤的 70%以上。最近的研究表明，透明质酸介导的运动受体（HMMR）可能是一种新的潜在肿瘤预后标志物。此外，越来越多的数据强调了 ceRNA 调控网络在多种人类恶性肿瘤中的重要作用。另一方面，HMMR 和神经胶质瘤中 ceRNA 网络的复杂性和行为特征尚不清楚。
从 TCGA、GTEx、GEO 和 CGGA 数据库中收集转录组表达数据。使用单变量和多变量 Cox 回归分析 HMMR 与临床变量之间的关系。Kaplan-Meier 方法用于评估 OS。分析 TCGA 数据并进行处理，将获得的相关结果用于进行 GO、GSEA 和 ssGSEA 分析。通过 miRWalk 和 StarBase 预测潜在相互作用的 miRNAs 和 lncRNAs。
HMMR 在神经胶质瘤组织中的表达明显高于正常组织。多变量分析显示，高 HMMR 表达是 TCGA 和 CGGA 中 OS 的独立预测指标。功能富集分析发现 HMMR 表达与核分裂和细胞周期有关。基于 ssGSEA 分析，各种免疫细胞中 HMMR 表达水平差异显著。生物信息学研究揭示了与神经胶质瘤预后相关的 HEELPAR-hsa-let-7i-5p-RRM2 ceRNA 网络。通过多种分析，证实了 HELLPAR/RRM2 轴对神经胶质瘤患者具有良好的预测性能。
本研究为 HMMR 的重要性提供了多层次、多方面的证据，并建立了与神经胶质瘤预后相关的 HMMR 相关 ceRNA（HEELPAR-hsa-let-7i-5p-RRM2）过表达网络。
