Pozzi C, di Pietro D, Halas G, Roig C, Salamini F
Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné -Weg, 10 50829 Cologne, Germany.
Heredity (Edinb). 2003 May;90(5):390-6. doi: 10.1038/sj.hdy.6800259.
The first step in positional gene cloning is the integration into available molecular maps of genetic loci for which mutant alleles exist. We report the placement of 29 barley developmental mutants on a restriction fragment length polymorphism-amplified fragment length polymorphism (RFLP-AFLP) map. The mapping procedure used homozygous mutant F(2) plants in an iterative process: once a mutant linked AFLP was found, primer combinations were successively selected to generate AFLP fragments more tightly linked to the mutant locus. The mutants considered were adp, als, aur-a1, aur-a2, br1, br2, bra-d7, cul3, cul5, cul15, cul16, den6, den8, dub1, hex-v3, hex-v4, int-c5, K, li, lig-a2, lk2, lk5, sld1, sld4, tr, trd, unc, uc2 and uz. The 29 mutant loci were linked to the closest molecular markers by distances ranging from 0 to 23 cM, with an average value of 3.8 cM away. Since the efficiency of the mapping procedure is a function of the density of molecular markers, the RFLP-AFLP map of Castiglioni et al was further integrated with new AFLPs using 87 doubled haploid lines derived from the barley cross Igri x Danilo. A total of 819 mapped AFLP marker loci are now available in the combined map.
位置基因克隆的第一步是将存在突变等位基因的遗传位点整合到可用的分子图谱中。我们报道了29个大麦发育突变体在限制性片段长度多态性-扩增片段长度多态性（RFLP-AFLP）图谱上的定位。定位过程在一个迭代过程中使用纯合突变F(2)植株：一旦发现与突变体连锁的AFLP，就相继选择引物组合以产生与突变位点连锁更紧密的AFLP片段。所考虑的突变体有adp、als、aur-a1、aur-a2、br1、br2、bra-d7、cul3、cul5、cul15、cul16、den6、den8、dub1、hex-v3、hex-v4、int-c5、K、li、lig-a2、lk2、lk5、sld1、sld4、tr、trd、unc、uc2和uz。这29个突变位点与最接近的分子标记的连锁距离在0到23厘摩之间，平均距离为3.8厘摩。由于定位过程的效率是分子标记密度的函数，因此使用来自大麦杂交种Igri×Danilo的87个双单倍体系，将Castiglioni等人的RFLP-AFLP图谱与新的AFLP进一步整合。现在在组合图谱中共有819个定位的AFLP标记位点。
