Pejic S, Kasapovic J, Cvetkovic D, Pajovic S B
Laboratory for Molecular Biology and Endocrinology, Vinca Institute of Nuclear Sciences, Belgrade, Yugoslavia.
Braz J Med Biol Res. 2003 May;36(5):579-86. doi: 10.1590/s0100-879x2003000500004. Epub 2003 Apr 22.
The sensitivity of copper,zinc (CuZn)- and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 micro g EB/100 g body weight in 0.1 ml olive oil/100 g body weight, 48 and 24 h before sacrifice. In females, EB treatment stimulated MnSOD activity on days 0 (66.1%), 8 (72.7%) and 15 (81.7%). In males, the stimulatory effect of EB on MnSOD activity on day 0 (113.6%) disappeared on day 8 and on days 15 and 45 it became inhibitory (40.3 and 30.5%, respectively). EB had no effect on the other age groups. The stimulatory effect of EB on CuZnSOD activity in newborn females (51.8%) changed to an inhibitory effect on day 8 (38.4%) and disappeared by day 45 when inhibition was detected again (48.7%). In males, the inhibitory effect on this enzyme was observed on days 0 (45.0%) and 15 (28.9%), and then disappeared until day 60 when a stimulatory effect was observed (38.4%). EB treatment had no effect on the other age groups. The sensitivity of MnSOD to estradiol differed significantly between sexes during the neonatal and prepubertal period, whereas it followed a similar pattern thereafter. The sensitivity of CuZnSOD to estradiol differed significantly between sexes during most of the study period. Regression analysis showed that the sensitivity of MnSOD to this estrogen tended to decrease similarly in both sexes, whereas the sensitivity of CuZnSOD showed a significantly different opposite tendency in female and male rats. These are the first reports indicating hormonal modulation of antioxidant enzyme activities related to the developmental process.
在出生后大鼠脑发育过程中，研究了铜锌（CuZn）-超氧化物歧化酶（SOD）和锰（Mn）-超氧化物歧化酶对苯甲酸雌二醇（EB）的敏感性。在处死前48小时和24小时，用0.1ml橄榄油/100g体重中含0.5μg EB/100g体重对0至75天不同年龄的雌雄大鼠进行皮下注射处理，然后从其大脑制备的样本中测量酶活性。在雌性大鼠中，EB处理在出生第0天（66.1%）、第8天（72.7%）和第15天（81.7%）刺激了MnSOD活性。在雄性大鼠中，EB对MnSOD活性在第0天的刺激作用（113.6%）在第8天消失，在第15天和第45天变为抑制作用（分别为40.3%和30.5%）。EB对其他年龄组无影响。EB对新生雌性大鼠CuZnSOD活性的刺激作用（51.8%）在第8天变为抑制作用（38.4%），到第45天消失，此时再次检测到抑制作用（48.7%）。在雄性大鼠中，在第0天（45.0%）和第15天（28.9%）观察到对该酶的抑制作用，然后直到第60天消失，此时观察到刺激作用（38.4%）。EB处理对其他年龄组无影响。在新生儿期和青春期前，MnSOD对雌二醇的敏感性在两性之间有显著差异，而此后遵循相似模式。在研究的大部分时间里，CuZnSOD对雌二醇的敏感性在两性之间有显著差异。回归分析表明，MnSOD对这种雌激素的敏感性在两性中都有类似的下降趋势，而CuZnSOD的敏感性在雌性和雄性大鼠中表现出明显不同的相反趋势。这些是首次表明与发育过程相关的抗氧化酶活性受到激素调节的报告。
