生存素蛋白在人类结直肠癌发生过程中的表达
Expression of survivin protein in human colorectal carcinogenesis.
作者信息
Lin Lian-Jie, Zheng Chang-Qing, Jin Yu, Ma Ying, Jiang Wei-Guo, Ma Tie
机构信息
Department of Gastroenterology of the 2nd Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China.
出版信息
World J Gastroenterol. 2003 May;9(5):974-7. doi: 10.3748/wjg.v9.i5.974.
AIM
To identify the role of survivin in colorectal carcinogenesis and the relationship between Survivin and histological differentiation grade of colorectal carcinoma.
METHODS
Immunohistochemical staining of survivin by using the monoclonal antibody was performed by the standard streptavidin-peroxidase (SP) technique for the 188 paraffin sections which included 30 normal colorectal mucosas, 41 adenomas with low grade dysplasia, 30 adenomas with high grade dysplasia, and 87 colorectal carcinomas which were classified as high, middle and low differentiated subgroups which included 33, 28, 26 cases respectively.
RESULTS
Expression of survivin was observed in the cytoplasm of adenoma with dysplasia and colorectal carcinoma cells. No immunoreactivity of survivin was seen in normal mucosas. The positive rate of survivin increased in the transition from normal mucosas to adenomas with low grade dysplasia to high grade dysplasia/ carcinomas (0.0 %, 31.7 %, 56.7 % and 63.2% respectively). But the difference between high grade dysplasia and carcinomas had no statistical significance. Positive rate was not related to histological differentiation grade of colorectal carcinoma. Moreover, there was no correlation between histological differentiation grade of colorectal carcinoma and immunoreactive intensity of survivin.
CONCLUSION
The expression of survivin is the essential event in the early stage of colorectal carcinogenesis and plays an important role in the transition sequence and it is not related to histological differentiation grade of colorectal carcinoma. It thus may provide a new diagnostic and therapeutic target in colorectal cancer.
目的
明确生存素在结直肠癌发生中的作用以及生存素与结直肠癌组织学分化程度之间的关系。
方法
采用标准链霉亲和素-过氧化物酶（SP）技术，使用单克隆抗体对188例石蜡切片进行生存素免疫组化染色，其中包括30例正常结直肠黏膜、41例低级别发育异常腺瘤、30例高级别发育异常腺瘤以及87例结直肠癌，后者又分为高分化、中分化和低分化亚组，分别包含33例、28例和26例。
结果
在发育异常腺瘤和结直肠癌细胞的细胞质中观察到生存素表达。在正常黏膜中未见到生存素的免疫反应性。从正常黏膜到低级别发育异常腺瘤再到高级别发育异常/癌，生存素的阳性率逐渐升高（分别为0.0%、31.7%、56.7%和63.2%）。但高级别发育异常与癌之间的差异无统计学意义。阳性率与结直肠癌的组织学分化程度无关。此外，结直肠癌的组织学分化程度与生存素的免疫反应强度之间无相关性。
结论
生存素的表达是结直肠癌发生早期的关键事件，在其转变过程中起重要作用，且与结直肠癌的组织学分化程度无关。因此，它可能为结直肠癌提供新的诊断和治疗靶点。
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