Zhang Xin, Luo Hesheng
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Oncol Lett. 2018 Mar;15(3):3313-3320. doi: 10.3892/ol.2017.7645. Epub 2017 Dec 19.
Lymphatic and hematogenous spread are the most common ways for tumors to metastasize. Angiogenesis is essential for tumor growth and metastasis. Vascular endothelial growth factor (VEGF) particularly VEGF-A is important in the process of angiogenesis. The current research has indicated that thalidomide (THD) may be able to inhibit angiogenesis, stimulate the activity of the immune system and inhibit the adherence of cancer cells to stromal cells. These changes may lead to suppression of tumor occurrence and development. To date, to the best of our knowledge, the effects of THD on colon cancer SW480 cells have not been reported. In the present study, the effects of THD and a combination of THD and oxaliplatin (L-OHP) on the proliferation of SW480 cells have been investigated. Furthermore, the expression of VEGF-A and hypoxia-inducible factor 1 (HIF-1) was analyzed using MTT assay, quantitative polymerase chain reaction and western blot analysis. The results indicated that THD was able to inhibit SW480 cells in dose-and-time dependent manner and inhibit the expression of VEGF-A and HIF-1α. Furthermore, treatment with THD and L-OHP had synergistic inhibitory effect, which may provide a novel treatment strategy for advanced colorectal cancer.
淋巴道转移和血行转移是肿瘤转移最常见的途径。血管生成对于肿瘤的生长和转移至关重要。血管内皮生长因子（VEGF），尤其是VEGF-A在血管生成过程中起重要作用。目前的研究表明，沙利度胺（THD）可能能够抑制血管生成，刺激免疫系统活性并抑制癌细胞与基质细胞的黏附。这些变化可能导致肿瘤发生和发展受到抑制。迄今为止，据我们所知，尚未报道THD对结肠癌SW480细胞的影响。在本研究中，研究了THD以及THD与奥沙利铂（L-OHP）联合应用对SW480细胞增殖的影响。此外，使用MTT法、定量聚合酶链反应和蛋白质印迹分析对VEGF-A和缺氧诱导因子1（HIF-1）的表达进行了分析。结果表明，THD能够以剂量和时间依赖性方式抑制SW480细胞，并抑制VEGF-A和HIF-1α的表达。此外，THD与L-OHP联合治疗具有协同抑制作用，这可能为晚期结直肠癌提供一种新的治疗策略。
