Zhang X, Wang X, Bai L, Li Z
Medical College, Shanghai Tiedao University, Shanghai 200070, China.
Wei Sheng Yan Jiu. 2000 May 30;29(3):166-7.
In order to investigate the effects of sodium selenite on the activity of glutathione peroxidase and the life-span of drosophila melanogaster, newly enclosed flies were divided randomly into 4 groups and fed with mediums containing different concentrations of sodium selenite. The activity of GSH-Px was determined by 5,5'-dithionbis (2-nitrobenzoic acid) (DTNB) photometric method. The life-span of drosophila was calculated after survival test. The results showed that the activity of GSH-Px in the female and male flies were increased by the increase of sodium selenite in the medium (P < 0.05). The average life-span and the average maximum life-span of flies in selenium groups were increased significantly as compared with control group (P < 0.05). The response of GSH-Px activity and life-span of male flies to selenium intake was better than that of females.
为了研究亚硒酸钠对果蝇体内谷胱甘肽过氧化物酶活性及寿命的影响，将刚羽化的果蝇随机分为4组，用含不同浓度亚硒酸钠的培养基喂养。采用5,5'-二硫代双(2-硝基苯甲酸)(DTNB)比色法测定谷胱甘肽过氧化物酶(GSH-Px)的活性。通过存活试验计算果蝇的寿命。结果表明，培养基中亚硒酸钠含量增加，雌雄果蝇体内GSH-Px的活性均升高(P<0.05)。与对照组相比，硒处理组果蝇的平均寿命和平均最长寿命均显著延长(P<0.05)。雄果蝇GSH-Px活性及寿命对硒摄入的反应比雌果蝇更好。
