Zhang Li, Ran Yun, Wu Mei, Wang Ling
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2009 Mar;38(2):144-7.
To explore the effect of ethanol on the life span and the activities of SOD and GSH-Px as well as the contents of MBA in Drosophila melanogaster.
Newly trapped and not mated 7200 flies were collected within 8 hours and separated by sex. 2400 flies were selected and randomly divided into six groups (0, 9, 27, 90, 270 and 8l0 mmol/L). Each group contained 400 flies, 200 male flies and 200 female flies. The numbers of dead flies were counted everyday and the culture mediums were replaced every 4 days until all flies died. Another 4800 flies were randomly divided into 4 groups (0, 27, 90 and 270 mmol/L). Each group contained 1200 flies, half male and half female. The activities of SOD and GSH-Px as well as the contents of MBA were detected at the days of 0th, 10th, 20th and 30th after ethanol exposure. Results The life span of Drosophila exposed to high dose ethanol reduced obviously. Activities of SOD and GSH-Px and contents of MDA of Drosophila exposed to ethanol reduced and enhanced with increasing of ethanol doses. And at the same doses (90 mmol/L and 270 mmol/L), activities of SOD and GSH-Px reduced, while contents of MDA elevated with increasing of exposure time. Exposed to high dose of ethanol at the days of 20 or 30, activities of SOD and GSH-Px and contents of MDA in Drosophila reduced and enhanced significantly in comparison with control group.
Ethanol could reduce the life span and the activities of SOD and GSH-Px, and enhance MBA content of Drosophila.
探讨乙醇对黑腹果蝇寿命、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性以及丙二醛（MDA）含量的影响。
在8小时内收集新捕获且未交配的7200只果蝇，并按性别分开。选取2400只果蝇，随机分为六组（0、9、27、90、270和810 mmol/L）。每组包含400只果蝇，其中雄蝇200只，雌蝇200只。每天统计死亡果蝇数量，每4天更换培养基，直至所有果蝇死亡。另外4800只果蝇随机分为4组（0、27、90和270 mmol/L）。每组包含1200只果蝇，雌雄各半。在乙醇暴露后的第0、10、20和30天检测SOD和GSH-Px活性以及MDA含量。结果：高剂量乙醇暴露的黑腹果蝇寿命明显缩短。乙醇暴露的果蝇SOD和GSH-Px活性以及MDA含量随乙醇剂量增加而降低和升高。在相同剂量（90 mmol/L和270 mmol/L）下，SOD和GSH-Px活性随暴露时间增加而降低，而MDA含量升高。在第20或30天暴露于高剂量乙醇时，与对照组相比，黑腹果蝇的SOD和GSH-Px活性以及MDA含量显著降低和升高。
乙醇可缩短黑腹果蝇寿命，降低SOD和GSH-Px活性，并增加MDA含量。
