Pinzón-Charry Alberto, Vernot Jean Paul, Rodríguez Raul, Patarroyo Manuel Elkin
Fundación Instituto de Immunología de Colombia, FIDIC, Universidad Nacional de Colombia, Bogotá, DC, Colombia.
J Med Primatol. 2003 Feb;32(1):31-8. doi: 10.1034/j.1600-0684.2003.00004.x.
The new world primate Aotus sp. has been recommended by the World Health Organization as a model for evaluation of malaria vaccine candidates, given its susceptibility to experimental infection with the human malaria parasites Plasmodium falciparum and P. vivax. The present study examined the in vitro proliferative response of peripheral blood mononuclear cells (PBMCs) isolated from Aotus monkeys, utilizing a wide range of mitogens. Results presented herein demonstrate that the in vitro proliferative response of PBMCs from the Aotus sp. is quite variable from monkey to monkey for each of the mitogens assessed. PBMCs from the Aotus monkey exhibited a delayed kinetic proliferative response and, particularly, a different sensitivity to proliferation in response to various concentrations of Phytohemagglutinin-P and favin lectins, the phorbol ester Phorbol myristate acetate and the calcium ionophore ionomycin. Altogether, our findings are consistent with the conclusion that the in vitro proliferative response of PBMCs from the Aotus differ in their activation requirements compared with PBMCs from humans.
鉴于新世界灵长类动物夜猴属（Aotus sp.）对人类疟原虫恶性疟原虫和间日疟原虫的实验性感染易感，世界卫生组织已推荐将其作为评估疟疾候选疫苗的模型。本研究利用多种有丝分裂原，检测了从夜猴分离的外周血单个核细胞（PBMC）的体外增殖反应。本文给出的结果表明，对于所评估的每种有丝分裂原，来自夜猴属的PBMC的体外增殖反应在不同猴子之间差异很大。来自夜猴的PBMC表现出延迟的动力学增殖反应，特别是对不同浓度的植物血凝素-P、法夫因凝集素、佛波酯十四酰佛波醇乙酯和钙离子载体离子霉素的增殖反应具有不同的敏感性。总之，我们的研究结果与以下结论一致：与人类PBMC相比，来自夜猴的PBMC的体外增殖反应在激活要求方面存在差异。
