Nehete Pramod N, Nehete Bharti P, Chitta Sriram, Williams Lawrence E, Abee Christian R
Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas;, Email:
Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Texas.
Comp Med. 2017 Feb 1;67(1):67-78.
Owl monkeys (Aotus nancymaae) are New World NHP that serve an important role in vaccine development and as a model for human disease conditions such as malaria. Despite the past contributions of this animal model, limited information is available about the phenotype and functional properties of peripheral blood lymphocytes in reference to sex and age. Using a panel of human antibodies and a set of standardized human immune assays, we identified and characterized various peripheral blood lymphocyte subsets, evaluated the immune functions of T cells, and analyzed cytokines relative to sex and age in healthy owl monkeys. We noted age- and sex-dependent changes in CD28+ (an essential T cell costimulatory molecule) and CD95+ (an apoptotic surface marker) T cells and various levels of cytokines in the plasma. In immune assays of freshly isolated peripheral blood mononuclear cells, IFNγ and perforin responses were significantly higher in female than in male monkeys and in young adults than in juvenile and geriatric groups, despite similar lymphocyte (particularly T cell) populations in these groups. Our current findings may be useful in exploring Aotus monkeys as a model system for the study of aging, susceptibility to infectious diseases, and age-associated differences in vaccine efficacy, and other challenges particular to pediatric and geriatric patients.
夜猴（Aotus nancymaae）是新大陆非人灵长类动物，在疫苗研发以及作为疟疾等人类疾病状况的模型方面发挥着重要作用。尽管这种动物模型过去有诸多贡献，但关于外周血淋巴细胞的表型和功能特性与性别和年龄相关的信息却很有限。我们使用一组人类抗体和一套标准化的人类免疫检测方法，对健康夜猴的外周血淋巴细胞亚群进行了鉴定和表征，评估了T细胞的免疫功能，并分析了与性别和年龄相关的细胞因子。我们注意到CD28 +（一种重要的T细胞共刺激分子）和CD95 +（一种凋亡表面标志物）T细胞以及血浆中各种细胞因子水平存在年龄和性别依赖性变化。在新鲜分离的外周血单核细胞的免疫检测中，尽管这些组中的淋巴细胞（特别是T细胞）群体相似，但雌性猴子和年轻成年猴子的IFNγ和穿孔素反应明显高于雄性猴子以及幼年和老年组。我们目前的研究结果可能有助于将夜猴作为研究衰老、对传染病的易感性以及疫苗效力的年龄相关差异，以及儿科和老年患者特有的其他挑战的模型系统进行探索。
