恶性疟原虫感染对夜猴脾脏和外周血淋巴细胞体外有丝分裂原反应的影响。

Effect of falciparum malaria infection on the in vitro mitogen responses of spleen and peripheral blood lymphocytes from owl monkeys.

作者信息

Taylor D W, Siddiqui W A

出版信息

Am J Trop Med Hyg. 1978 Jul;27(4):738-42. doi: 10.4269/ajtmh.1978.27.738.

DOI:10.4269/ajtmh.1978.27.738

Abstract

The response of spleen and peripheral blood lymphocytes (PBL) to mitogen stimulation with phytohemagglutinin (PHA), Concanavalin A (ConA), and pokeweed mitogen (PWM) was determined for owl monkeys (Aotus trivirgatus) experimentally infected with the Vietnam-Oak Knoll (FVO) and the Uganda-Palo Alto (FUP) strains of Plasmodium falciparum. PBL from Panamanian Aotus monkeys with less than 25% FVO infection responded normally to mitogen stimulation; however, increased parasitemia of 25--50% resulted in a significant suppression of ConA responsiveness. Colombian Aotus monkeys infected with the PUF strain also developed a suppression to ConA stimulation but with a lower parasitemia (10--25%). When the parasitemia became greater than 50% in these animals, PHA, ConA, and PWM responses were significantly decreased in cultures of PBL. Spleen cells from all acutely infected Aotus monkeys were suppressed to PHA and ConA, but not PWM stimulation. Changes in mitogen responsiveness of experimentally infected Aotus monkeys are similar to those reported for humans with acute falciparum malaria.

摘要

对于实验感染了越南-橡树岭（FVO）和乌干达-帕洛阿尔托（FUP）恶性疟原虫株的夜猴（Aotus trivirgatus），测定了脾脏和外周血淋巴细胞（PBL）对植物血凝素（PHA）、刀豆球蛋白A（ConA）和商陆有丝分裂原（PWM）的有丝分裂原刺激的反应。来自巴拿马夜猴且FVO感染率低于25%的PBL对有丝分裂原刺激反应正常；然而，寄生虫血症增加至25%-50%会导致ConA反应性显著抑制。感染PUF株的哥伦比亚夜猴对ConA刺激也产生了抑制，但寄生虫血症较低（10%-25%）。当这些动物的寄生虫血症超过50%时，PBL培养物中PHA、ConA和PWM反应显著降低。所有急性感染夜猴的脾细胞对PHA和ConA刺激均受到抑制，但对PWM刺激未受抑制。实验感染夜猴的有丝分裂原反应性变化与急性恶性疟患者报道的情况相似。