通过荧光原位杂交技术对孕妇阴道标本中的无乳链球菌进行快速筛查。
Rapid screening for Streptococcus agalactiae in vaginal specimens of pregnant women by fluorescent in situ hybridization.
作者信息
Artz Laura A, Kempf Volkhard A J, Autenrieth Ingo B
机构信息
Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Eberhard-Karls-Universität Tübingen, D-72076 Tübingen, Germany.
出版信息
J Clin Microbiol. 2003 May;41(5):2170-3. doi: 10.1128/JCM.41.5.2170-2173.2003.
Abstract
Group B streptococci (GBS) are the most frequent pathogens in neonates with sepsis. A rapid screening method is required to identify carriage of GBS in pregnant women at the time of delivery. In order to detect GBS in vaginal specimens, the efficiency of the standard culture versus fluorescent in situ hybridization (FISH) was investigated. In 258 examined vaginal specimens, FISH identified 58 of the 59 GBS-positive samples (98.3%), whereas by means of standard culture only 38 specimens were positive (64.4%). We recommend FISH as a rapid, specific, highly sensitive screening technique for the detection of GBS in pregnant women at delivery.
摘要
B族链球菌（GBS）是新生儿败血症中最常见的病原体。需要一种快速筛查方法来在分娩时识别孕妇是否携带GBS。为了检测阴道标本中的GBS，研究了标准培养法与荧光原位杂交（FISH）法的效率。在258份检测的阴道标本中，FISH在59份GBS阳性样本中识别出58份（98.3%），而通过标准培养法只有38份标本呈阳性（64.4%）。我们推荐FISH作为一种快速、特异、高度灵敏的筛查技术，用于在分娩时检测孕妇中的GBS。
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