通过实时PCR对人疱疹病毒6B进行灵敏定量分析。
A Sensitive Quantification of HHV-6B by Real-time PCR.
作者信息
ØSter Bodil, Höllsberg Per
机构信息
Department of Medical Microbiology and Immunology, Bartholin Building, University of Aarhus. DK-8000 Aarhus. Denmark.
出版信息
Biol Proced Online. 2002 Dec 9;4:88-93. doi: 10.1251/bpo38.
Human herpesvirus (HHV)-6B is a pathogen causing latent infection in virtually all humans. Nevertheless, the interaction of HHV-6B with its host cells is poorly understood. Although HHV-6B is approximately 90% homologous to HHV-6A, it expresses certain B-specific genes. In order to quantify the amount of expressed viral mRNA we have developed a method using real-time PCR on a LightCycler instrument. Here we describe an assay for the detection of the HHV-6B B6 mRNA, but our approach can easily be extended to involve other mRNAs. This method is useful during the study of HHV-6B biology and offers reliable and reproducible, quantitative detection of viral mRNA below the attomol range.
人类疱疹病毒6B型（HHV - 6B）是一种几乎能在所有人中引起潜伏感染的病原体。然而，HHV - 6B与其宿主细胞之间的相互作用却鲜为人知。尽管HHV - 6B与HHV - 6A大约有90%的同源性，但它会表达某些B特异性基因。为了定量表达的病毒mRNA的量，我们开发了一种在LightCycler仪器上使用实时PCR的方法。在此，我们描述一种用于检测HHV - 6B B6 mRNA的测定方法，但我们的方法可以很容易地扩展到检测其他mRNA。这种方法在HHV - 6B生物学研究中很有用，并且能够可靠、可重复地定量检测低于阿托摩尔范围的病毒mRNA。
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