Ren Jun, Wold Loren E
Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota School of Medicine. 501 N. Columbia Road, Grand Forks, ND 58203. USA.
Biol Proced Online. 2001 Dec 11;3:43-53. doi: 10.1251/bpo22.
Isolated adult cardiac ventricular myocytes have been a useful model for cardiovascular research for more than 20 years. With the recent advances in cellular physiology and transgenic techniques, direct measurement of isolated ventricular myocyte mechanics is becoming an increasingly important technique in cardiac physiology that provides fundamental information on excitation-contraction coupling of the heart, either in drug intervention or pathological states. The goal of this article is to describe the isolation of ventricular myocytes from both rats and mice, and the use of real-time beat-to-beat simultaneous recording of both myocyte contraction and intracellular Ca(2+) transient.
20多年来，分离的成年人心室肌细胞一直是心血管研究的有用模型。随着细胞生理学和转基因技术的最新进展，直接测量分离的心室肌细胞力学正成为心脏生理学中一项越来越重要的技术，它能提供关于心脏兴奋-收缩偶联的基本信息，无论是在药物干预还是病理状态下。本文的目的是描述从大鼠和小鼠中分离心室肌细胞，以及实时逐搏同步记录肌细胞收缩和细胞内Ca(2+)瞬变的方法。
