Kobayashi Takashi, Hattori Shunji, Shinkai Hiroshi
Department of Dermatology/Clinical Biology of Extracellular Matrix (F7), Chiba University School of Medicine, Chiba, Japan.
Acta Derm Venereol. 2003;83(2):105-7. doi: 10.1080/00015550310007436.
To investigate the pathophysiological role of fibroblasts in tissue metabolism, we analysed gelatin-degrading enzymes secreted from skin cells in culture. Using a gelatin-zymography method, matrix metalloproteinase (MMP)-2 was constitutively secreted from fibroblasts, whereas both MMP-2 and MMP-9 were secreted from keratinocytes. MMP-9 expression by fibroblasts was induced by the addition of transforming growth factor (TGF)-beta1 or tumour necrosis factor (TNF)-alpha to the cultures. Moreover, TGF-beta1, TNF-alpha, or epidermal growth factor augmented the secretion of MMP-2 from fibroblasts. These results indicate that MMP-9 together with MMP-2 secreted from fibroblasts could play important roles in tissue metabolism, including cytokine-induced inflammation.
为了研究成纤维细胞在组织代谢中的病理生理作用，我们分析了培养的皮肤细胞分泌的明胶降解酶。使用明胶酶谱法，基质金属蛋白酶（MMP）-2由成纤维细胞组成性分泌，而MMP-2和MMP-9均由角质形成细胞分泌。通过向培养物中添加转化生长因子（TGF）-β1或肿瘤坏死因子（TNF）-α诱导成纤维细胞表达MMP-9。此外，TGF-β1、TNF-α或表皮生长因子可增强成纤维细胞分泌MMP-2。这些结果表明，MMP-9与成纤维细胞分泌的MMP-2一起可能在包括细胞因子诱导的炎症在内的组织代谢中发挥重要作用。
