Termén S, Tollin M, Olsson B, Svenberg T, Agerberth B, Gudmundsson G H
Microbiology and Tumor Biology Center, Nobels väg 16, Karolinska Institutet, 17177 Stockholm, Sweden.
Cell Mol Life Sci. 2003 Mar;60(3):536-49. doi: 10.1007/s000180300045.
A database search identified a rat cDNA clone which phylogenetic analysis revealed to encode a cathelicidin most similar to mouse cathelicidin CRAMP. The analysis also showed that the evolutionary pattern of the cathelicidin family is lineage specific. The rat cathelicidin is called rCRAMP. Its peptide was isolated from granulocytes, and determined to be 43 amino acids long by mass spectrometry and N-terminal sequencing. Synthetic rCRAMP had antimicrobial activity. The expression of rCRAMP was investigated by reverse-transcriptase polymerase chain reaction followed by Southern hybridization and by Western blot analysis. rCRAMP was identified in granulocytes, thymus, testis, lung, mouth mucosa, tongue, oesophagus, colon, caecum and small intestine, a distribution similar to cathelicidins of mouse and human. The rat is a small laboratory animal with additional disease models available compared to the mouse. Our results open up the possibility to use the rat as a model system to study responses connected to cathelicidin expression in health and disease.
一项数据库搜索鉴定出一个大鼠cDNA克隆，系统发育分析显示该克隆编码一种与小鼠cathelicidin CRAMP最为相似的cathelicidin。分析还表明，cathelicidin家族的进化模式具有谱系特异性。大鼠cathelicidin被称为rCRAMP。其肽段从粒细胞中分离出来，通过质谱分析和N端测序确定其长度为43个氨基酸。合成的rCRAMP具有抗菌活性。通过逆转录聚合酶链反应，随后进行Southern杂交和蛋白质印迹分析，对rCRAMP的表达进行了研究。在粒细胞、胸腺、睾丸、肺、口腔黏膜、舌、食管、结肠、盲肠和小肠中鉴定出rCRAMP，其分布与小鼠和人类的cathelicidin相似。大鼠是一种小型实验动物，与小鼠相比有更多可用的疾病模型。我们的结果为将大鼠用作模型系统来研究与健康和疾病中cathelicidin表达相关的反应开辟了可能性。
