Kong Jian, Gouras Peter
Department of Ophthalmology, Columbia University, New York, NY 10032, USA.
Doc Ophthalmol. 2003 May;106(3):239-42. doi: 10.1023/a:1022988332578.
To study the effect of body temperature on the murine electroretinogram (ERG).
The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37 degrees C.
ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37 degrees C. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 microV/degree. At 30 degrees C, maximum b-wave amplitude was about 400 microV; at 37 degrees C it was about 1000 microV. A change in body temperature produced a rapid change in ERG amplitude.
The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.
研究体温对小鼠视网膜电图（ERG）的影响。
使用盐水灯芯电极记录暗适应小鼠在频闪闪光刺激下诱发的角膜ERG，同时连续测量直肠温度。将小鼠置于一个圆柱形的盘管内，水从温度控制浴中循环通过该盘管。小鼠的体温在30至37摄氏度之间逐步变化。
在30至37摄氏度的体温范围内记录到了大致正常形态的ERG。a波和b波的最大振幅随温度呈线性变化。b波振幅的变化率约为100微伏/摄氏度。在30摄氏度时，b波最大振幅约为400微伏；在37摄氏度时约为1000微伏。体温变化会使ERG振幅迅速改变。
小鼠ERG对温度变化非常敏感。为了随时间准确监测ERG，连续记录体温至关重要。
