Mizota Atsushi, Adachi-Usami Emiko
Department of Ophthalmology and Visual Science, Graduate School of Medicine, Chiba University, Japan.
Invest Ophthalmol Vis Sci. 2002 Dec;43(12):3754-7.
To investigate the ERG alterations induced by changes in body temperature in mice.
Three-week-old BALB/c mice were used. Rectal temperature was measured with a digital thermometer and taken as the body temperature. In experiment 1, the body temperature was kept at 33 degrees C, and the ERGs elicited by a constant stimulus intensity were recorded every 5 minutes. In experiment 2, the body temperature was lowered in five steps from 38 degrees C to 33 degrees C, 28 degrees C, 23 degrees C, and 18 degrees C. At each body temperature, ERGs elicited by different stimulus intensities were recorded.
In experiment 1, the mean amplitudes and implicit times of both the a- and b-waves did not change significantly. In experiment 2, the amplitude of both the a- and b-waves decreased significantly with a decrease in body temperature, and the implicit times of the a- and b-waves were prolonged with a decrease in body temperature.
Body temperature greatly affects the amplitude and timing of the ERG. Great care must be taken to maintain as normal a body temperature as possible when using the ERG to evaluate the retina, especially in small animals such as mice.
研究小鼠体温变化引起的视网膜电图（ERG）改变。
使用3周龄的BALB/c小鼠。用数字温度计测量直肠温度并将其作为体温。在实验1中，体温保持在33℃，每隔5分钟记录恒定刺激强度诱发的ERG。在实验2中，体温分五步从38℃降至33℃、28℃、23℃和18℃。在每个体温下，记录不同刺激强度诱发的ERG。
在实验1中，a波和b波的平均振幅及隐含时间均无明显变化。在实验2中，a波和b波的振幅均随体温降低而显著下降，a波和b波的隐含时间随体温降低而延长。
体温对ERG的振幅和时间有很大影响。在使用ERG评估视网膜时，尤其是在小鼠等小动物中，必须格外小心以保持尽可能正常的体温。
