糖基磷脂酰肌醇（GPI）锚定肽的首次全合成。

First total synthesis of a GPI-anchored peptide.

作者信息

Xue Jie, Shao Ning, Guo Zhongwu

机构信息

Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-7078, USA.

出版信息

J Org Chem. 2003 May 16;68(10):4020-9. doi: 10.1021/jo034213t.

DOI:10.1021/jo034213t

PMID:12737586

Abstract

A GPI-anchored dipeptide of sperm CD52 antigen was prepared through a convergent synthesis. First, the dipeptide with its C-terminus free and the GPI with its nonreducing end phosphoethanolamine bearing a free amino group were synthesized separately. Then, the two building blocks were coupled with use of EDC/HOBt as the condensation reagent. Finally, the GPI-anchored peptide was deprotected to give the target molecule 1.

摘要

通过汇聚合成法制备了精子CD52抗原的糖基磷脂酰肌醇（GPI）锚定二肽。首先，分别合成了C端游离的二肽和非还原端磷酸乙醇胺带有游离氨基的GPI。然后，使用1-乙基-3-(3-二甲基氨基丙基)碳二亚胺盐酸盐（EDC）/1-羟基苯并三唑（HOBt）作为缩合试剂将这两个结构单元偶联。最后，对GPI锚定肽进行脱保护以得到目标分子1。