糖基磷脂酰肌醇锚定糖肽的酶促化学合成。
Chemoenzymatic synthesis of glycosylphosphatidylinositol-anchored glycopeptides.
机构信息
Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA.
出版信息
Chem Commun (Camb). 2010 Aug 21;46(31):5773-4. doi: 10.1039/c0cc00828a. Epub 2010 Jun 24.
Abstract
MUC1 glycopeptide was efficiently coupled to glycosylphosphatidylinositol (GPI) derivatives by sortase A (SrtA), verifying that SrtA can accept sterically hindered glycopeptide as substrate for ligation with GPIs. This work has established a practical method for the chemoenzymatic synthesis of GPI-linked glycopeptides.
摘要
MUC1 糖肽通过 Sortase A（SrtA）有效地与糖基磷脂酰肌醇（GPI）衍生物偶联，验证了 SrtA 可以接受空间位阻的糖肽作为与 GPI 连接的缀合的底物。这项工作为 GPI 连接的糖肽的化学酶合成建立了一种实用的方法。
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