Sortase A 介导的化学酶法合成复杂糖基磷脂酰肌醇锚定蛋白。
Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein.
机构信息
Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, USA.
出版信息
Chem Commun (Camb). 2013 Dec 25;49(99):11689-91. doi: 10.1039/c3cc47229a.
Abstract
Green fluorescent protein and a glycosylphosphatidylinositol (GPI) anchor containing the common core structure and a lipid chain were synthesized and then coupled together in the promotion of bacterial sortase A (SrtA), which was the first example for the synthesis of a full-size GPI-anchored protein by SrtA, demonstrating that this can be a generally useful method for GPI-anchored protein synthesis.
摘要
绿色荧光蛋白和一种含有共同核心结构和脂质链的糖基磷脂酰肌醇（GPI）锚被合成，然后在细菌 sortase A（SrtA）的促进下连接在一起，这是 SrtA 合成全长 GPI 锚定蛋白的第一个例子，证明这是一种用于 GPI 锚定蛋白合成的通用方法。
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