用于快速检测粪便样本中霍乱弧菌O1和O139的一步免疫层析试纸条检测法。
One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples.
作者信息
Nato F, Boutonnier A, Rajerison M, Grosjean P, Dartevelle S, Guénolé A, Bhuiyan N A, Sack D A, Nair G B, Fournier J M, Chanteau S
机构信息
Institut Pasteur, Paris, France.
出版信息
Clin Diagn Lab Immunol. 2003 May;10(3):476-8. doi: 10.1128/cdli.10.3.476-478.2003.
Abstract
We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.
摘要
我们描述了一种基于使用金颗粒检测脂多糖的霍乱弧菌O1和O139快速诊断测试的开发和评估。特异性在84%至100%之间。当用在马达加斯加和孟加拉国获得的粪便样本进行评估时，试纸条的灵敏度范围为94.2%至100%。该试纸条可为流行病学调查提供一种简单的工具。
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