Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Asian Pac J Cancer Prev. 2021 Jun 1;22(6):1875-1881. doi: 10.31557/APJCP.2021.22.6.1875.
The main objectives of this study were to investigate the detection rate of high-risk human papillomavirus types 16 and 18 (high-risk HPV16/18) in oral potentially malignant disorders (OPMDs) including oral leukoplakia (OL) and oral lichen planus (OLP) in a Thai population and their associations with demographic, risk habits, and clinicopathologic features.
Paraffin-embedded formalin-fixed specimens from 101 OL and 59 OLP patients with patients' demographic, risk habits, and clinicopathologic data were collected. Conventional qualitative polymerase chain reaction was used to detect high-risk HPV16/18 DNA. Associations between high-risk HPV type 16/18 and demographic, clinicopathologic, risk factors (tobacco and alcohol uses) of OPMDs were analysed by Chi-square or Fisher's exact test. The results with p value less than 0.05 were considered statistically significant.
HPV16/18 DNA was found in both OL and OLP groups with the detection rate of 19.8% and 18.6%, respectively. Approximately 90% of high-risk HPV were HPV18 subtype. Additionally, in OL group, high-risk HPV was found more frequently in patients with moderate/severe dysplasia than that in mild dysplasia. Interestingly, in OLP group, high-risk HPV was only detected in atrophic/ulcerative subtypes. None of risk factors was associated with high-risk HPV.
Approximately 19% of OPMDs were HPV16/18-positive. HPV18 DNA was predominantly detected in both OL and OLP patients (90%). Additionally, the detection rate of high-risk HPV was higher in more severe dysplastic cases of OL and more clinically severe cases of OLP.
本研究的主要目的是调查高危型人乳头瘤病毒 16 型和 18 型（高危 HPV16/18）在包括口腔白斑（OL）和口腔扁平苔藓（OLP）在内的泰国人口口腔潜在恶性疾病（OPMDs）中的检出率及其与人口统计学、风险习惯和临床病理特征的关系。
收集了 101 例 OL 和 59 例 OLP 患者的石蜡包埋福尔马林固定标本，包括患者的人口统计学、风险习惯和临床病理数据。采用常规定性聚合酶链反应检测高危 HPV16/18 DNA。采用卡方或 Fisher 精确检验分析高危 HPV 型 16/18 与 OPMD 的人口统计学、临床病理、危险因素（烟草和酒精使用）之间的关系。p 值小于 0.05 被认为具有统计学意义。
OL 和 OLP 组均检测到 HPV16/18 DNA，检出率分别为 19.8%和 18.6%。大约 90%的高危 HPV 为 HPV18 亚型。此外，在 OL 组中，高危 HPV 在中重度异型增生患者中比轻度异型增生患者更常见。有趣的是，在 OLP 组中，高危 HPV 仅在萎缩/溃疡性亚型中检测到。没有一个危险因素与高危 HPV 相关。
大约 19%的 OPMD 为 HPV16/18 阳性。OL 和 OLP 患者中主要检测到 HPV18 DNA（90%）。此外，OL 中高危 HPV 的检出率在异型增生程度较重的病例和 OLP 中临床严重程度较重的病例中较高。
