Yang Z, Yuan H, Tong W, Zou P, Chen W, Zhang X
Institute of Materials Science and Technology, Sichuan University, Chengdu, People's Republic of China.
Biomaterials. 1996 Nov;17(22):2131-7. doi: 10.1016/0142-9612(96)00044-0.
Synthetic porous calcium phosphate ceramics (HA/TCP) were implanted intramuscularly and subcutaneously in dogs, pigs, goats, rabbits and rats, designed to make a comparative study of the host tissue responses to porous HA/TCP ceramics in different kinds of animals. Specimens were harvested at 15, 30, 45, 60, 90 and 120 days after implantation. Decalcified and undecalcified sections were made and examined by light microscopy. Obvious bone formation could be detected in some specimens harvested from dogs and pigs after 45 days intramuscular implantation or after 60 days subcutaneous implantation. At days 90 and 120, an extensive amount of bone formed in all specimens implanted in dogs and pigs. However, no histologically detectable bone formation was observed in any specimen implanted intramuscularly and subcutaneously in goats, rabbits and rats until 120 days. It is demonstrated from this finding that the synthetic porous calcium phosphate ceramics are capable of inducing osteogenesis when implanted in non-bony sites, but this ability varies between different kinds of animals. Earlier periods of observation in specimens harvested from dogs showed that bone differentiation in the pore regions of the ceramics follows a complex process involving invasion of the fibrovascular connective tissues at day 15, appearance of polymorphic mesenchymal cells near the invading vasculature and at the interface with the ceramics at day 30, differentiation of osteoblasts and formation of bone matrix in direct contact with the surface of the ceramics at day 45, and finally remodelling of the fibrous connective tissue into an extensive amount of bone at days 60, 90 and 120.
将合成多孔磷酸钙陶瓷（HA/TCP）分别肌肉内和皮下植入犬、猪、山羊、兔和大鼠体内，旨在对不同种类动物对多孔HA/TCP陶瓷的宿主组织反应进行比较研究。在植入后15、30、45、60、90和120天采集标本。制作脱钙和未脱钙切片并通过光学显微镜检查。肌肉内植入45天后或皮下植入60天后，从犬和猪采集的一些标本中可检测到明显的骨形成。在90天和120天时，植入犬和猪的所有标本中都形成了大量的骨。然而，直到120天，在山羊、兔和大鼠肌肉内和皮下植入的任何标本中均未观察到组织学上可检测到的骨形成。从这一发现可以证明，合成多孔磷酸钙陶瓷植入非骨部位时能够诱导成骨，但这种能力在不同种类动物之间有所不同。对从犬采集的标本进行早期观察表明，陶瓷孔隙区域的骨分化遵循一个复杂的过程，包括第15天纤维血管结缔组织的侵入，第30天在侵入血管附近和与陶瓷界面处出现多形性间充质细胞，第45天成骨细胞分化并形成与陶瓷表面直接接触的骨基质，最后在第60、90和120天纤维结缔组织重塑为大量的骨。
