Lepock James R
Department of Medical Biophysics, University of Toronto, and Ontario Cancer Institute, Princess Margaret Hospital, 610 University Avenue, Toronto, Ontario M5G 2M9 Canada.
Int J Hyperthermia. 2003 May-Jun;19(3):252-66. doi: 10.1080/0265673031000065042.
The specific mechanism of cell killing by hyperthermia is unknown, but the high activation energy of cell killing and other responses to hyperthermia suggest that protein denaturation is the rate-limiting step. Protein denaturation can be directly monitored by differential scanning calorimetry and in general there is a good correlation between protein denaturation and cellular response. Approximately 5% denaturation is necessary for detectable killing. Protein denaturation leads to the aggregation of both denatured and native protein with multiple effects on cellular function.
热疗导致细胞死亡的具体机制尚不清楚，但细胞死亡及其他对热疗反应的高活化能表明蛋白质变性是限速步骤。蛋白质变性可通过差示扫描量热法直接监测，一般来说，蛋白质变性与细胞反应之间存在良好的相关性。大约5%的变性是可检测到的细胞死亡所必需的。蛋白质变性会导致变性蛋白和天然蛋白聚集，对细胞功能产生多种影响。
