Orhan Ilkay, Nasim Shama, Sener Bilge, Ayanoglu Filiz, Ozgüven Mensure, Choudhary M Iqbal, ur-Rahman Atta
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
Phytother Res. 2003 May;17(5):575-7. doi: 10.1002/ptr.1169.
In vitro biological activities including bactericidal, fungicidal and insecticidal activities as well as phytotoxicity and brine shrimp toxicity of the petroleum ether, chloroform and ethyl acetate extracts of Iris germanica L. were determined. The bactericidal activity of the extracts was assayed by the agar well diffusion test. In the fungicidal test, the agar tube dilution method was used. The insecticidal activity was determined by the exposure method. The toxicity of the extracts was evaluated by the phytotoxicity test as well as the brine shrimp toxicity test. The chloroform and ethyl acetate extracts of I. germanica rhizomes exhibited bactericidal activity, while the petroleum ether extract did not exhibit any bactericidal, fungicidal and insecticidal activities. It was also inactive in the brine shrimp toxicity test, whereas it showed significant phytotoxicity against the plant Lemna aequinoctialis Welv. Two known isoflavones were isolated from the chloroform extract of the plant.
测定了德国鸢尾石油醚、氯仿和乙酸乙酯提取物的体外生物活性，包括杀菌、杀真菌和杀虫活性以及植物毒性和卤虫毒性。提取物的杀菌活性通过琼脂孔扩散试验测定。在杀真菌试验中，使用琼脂管稀释法。杀虫活性通过暴露法测定。提取物的毒性通过植物毒性试验和卤虫毒性试验进行评估。德国鸢尾根茎的氯仿和乙酸乙酯提取物表现出杀菌活性，而石油醚提取物未表现出任何杀菌、杀真菌和杀虫活性。它在卤虫毒性试验中也无活性，而对浮萍表现出显著的植物毒性。从该植物的氯仿提取物中分离出两种已知的异黄酮。
