Ciegler A, Beckwith A C, Jackson L K
Appl Environ Microbiol. 1976 May;31(5):664-7. doi: 10.1128/aem.31.5.664-667.1976.
The mean lethal dose of patulin for the chicken embryo injected in the air cell before incubation was determined to be 68.7 mug and that for the 4-day-old embryo was 2.35 mug. Both patulin (1 to 2 mug/egg) and the reaction mixture between patulin and cysteine (15 to 150 mug of patulin equivalents) were teratogenic to the chicken embryo. At least two ninhydrin-negative and four ninhydrin-positive products were formed during the latter reaction. Our explanation of the reaction mechanism remains to be elaborated.
在孵化前向鸡胚气室注射展青霉素的平均致死剂量测定为68.7微克，4日龄胚胎的平均致死剂量为2.35微克。展青霉素（1至2微克/蛋）以及展青霉素与半胱氨酸的反应混合物（15至150微克展青霉素当量）对鸡胚均具有致畸性。在后一种反应过程中至少形成了两种茚三酮阴性产物和四种茚三酮阳性产物。我们对该反应机制的解释仍有待完善。
