Khan Naghma, Sharma Sonia, Sultana Sarwat
Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard, Hamdard University, New Delhi 110 062, India.
Hum Exp Toxicol. 2003 Apr;22(4):193-203. doi: 10.1191/0960327103ht349oa.
Potassium bromate (KBrO3) is a potent nephrotoxic agent. In this paper, we report the chemopreventive effect of Nigella sativa (black cumin) on KBrO3-mediated renal oxidative stress, toxicity and tumor promotion response in rats. KBrO3 (125 mg/kg body weight, intraperitoneally) enhances lipid peroxidation, gamma-glutamyl transpeptidase, hydrogen peroxide and xanthine oxidase with reduction in the activities of renal antioxidant enzymes and renal glutathione content. A marked increase in blood urea nitrogen and serum creatinine has also been observed. KBrO3 treatment also enhances ornithine decarboxylase (ODC) activity and [3H] thymidine incorporation into renal DNA. Prophylaxis of rats orally with Nigella sativa extract (50 mg/kg body weight and 100 mg/kg body weight) resulted in a significant decrease in renal microsomal lipid peroxidation (P < 0.001), gamma-glutamyl transpeptidase (P < 0.001), H2O2 (P < 0.001) and xanthine oxidase (P < 0.05). There was significant recovery of renal glutathione content (P < 0.01) and antioxidant enzymes (P < 0.001). There was also reversal in the enhancement of blood urea nitrogen, serum creatinine, renal ODC activity and DNA synthesis (P < 0.001). Data suggest that Nigella sativa is a potent chemopreventive agent and may suppress KBrO3-mediated renal oxidative stress, toxicity and tumour promotion response in rats.
溴酸钾(KBrO3)是一种强效肾毒性剂。在本文中,我们报告了黑种草(黑孜然)对溴酸钾介导的大鼠肾脏氧化应激、毒性和肿瘤促进反应的化学预防作用。溴酸钾(125毫克/千克体重,腹腔注射)会增强脂质过氧化、γ-谷氨酰转肽酶、过氧化氢和黄嘌呤氧化酶,同时降低肾脏抗氧化酶的活性和肾脏谷胱甘肽含量。还观察到血尿素氮和血清肌酐显著增加。溴酸钾处理还会增强鸟氨酸脱羧酶(ODC)活性以及[3H]胸苷掺入肾脏DNA。给大鼠口服黑种草提取物(50毫克/千克体重和100毫克/千克体重)进行预防,导致肾脏微粒体脂质过氧化(P < 0.001)、γ-谷氨酰转肽酶(P < 0.001)、过氧化氢(P < 0.001)和黄嘌呤氧化酶(P < 0.05)显著降低。肾脏谷胱甘肽含量(P < 0.01)和抗氧化酶(P < 0.001)有显著恢复。血尿素氮、血清肌酐、肾脏ODC活性和DNA合成的增强也出现了逆转(P < 0.001)。数据表明,黑种草是一种强效化学预防剂,可能抑制溴酸钾介导的大鼠肾脏氧化应激、毒性和肿瘤促进反应。