在“99%二甲基亚砜”梯度中对核糖体核糖核酸和不均一核糖核酸进行离心时出现的假象。
Artifacts in the centrifugation of ribosomal and heterogenous ribonucleic acid in "99%-dimethyl sulphoxide" gradients.
作者信息
Maxwell I H
出版信息
Biochem J. 1976 Feb 1;153(2):509-12. doi: 10.1042/bj1530509.
Abstract
Increasing the total quantity of RNA located on 99%-dimethyl sulphoxide gradients profoundly altered the sedimentation pattern of heterogenous RNA and (to a smaller extent) that of rRNA. Aggregation of small quantities of rRNA with fast-sedimenting heterogenous RNA was also found to occur in these gradients.
摘要
增加位于99%二甲基亚砜梯度上的RNA总量，会显著改变异源RNA的沉降模式，并（在较小程度上）改变rRNA的沉降模式。在这些梯度中还发现，少量rRNA会与快速沉降的异源RNA发生聚集。
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