Aust Gabriela, Hamann Jörg, Schilling Nicole, Wobus Manja
Institute of Anatomy, University of Leipzig, Ph.-Rosenthal-Strasse 55, 04103 Leipzig, Germany.
Virchows Arch. 2003 Jul;443(1):32-7. doi: 10.1007/s00428-003-0812-4. Epub 2003 May 22.
EMR2 and CD97, members of the epidermal growth factor (EGF)-TM7 family, show a very high homology. CD97, whose expression is closely related to clinical tumor stage in colorectal carcinomas, potentially functions as an adhesion molecule. Nothing is known about the presence of EMR2 in these tumors. We systematically examined the expression of EMR2 in colorectal carcinoma cell lines and adenocarcinomas. Of 18 cell lines, 10 were only slightly positive for EMR2 according to flow cytometry. Various EMR2 splice variants, including a new isoform, have been detected at the mRNA level. EMR2 expression did not correlate with in vitro migration or invasion capacity of the cell lines. Normal colorectal epithelial cells were EMR2 negative. In contrast to CD97, which is found in most colorectal adenocarcinomas, only 8 of 81 of these tumors expressed EMR2. No correlation was found between EMR2 expression and clinicopathological parameters of the tumors. In summary, a significant but low number of colorectal carcinomas are positive for EMR2, indicating different roles for this molecule and CD97 in these tumors.
表皮生长因子（EGF）-TM7家族成员EMR2和CD97具有很高的同源性。CD97的表达与结直肠癌的临床肿瘤分期密切相关，可能作为一种黏附分子发挥作用。关于这些肿瘤中EMR2的存在情况尚无相关报道。我们系统地检测了EMR2在结直肠癌细胞系和腺癌中的表达。在18个细胞系中，根据流式细胞术检测，10个细胞系仅呈微弱的EMR2阳性。在mRNA水平检测到了多种EMR2剪接变体，包括一种新的异构体。EMR2的表达与细胞系的体外迁移或侵袭能力无关。正常结直肠上皮细胞EMR2呈阴性。与大多数结直肠腺癌中存在的CD97不同，在81个此类肿瘤中只有8个表达EMR2。未发现EMR2表达与肿瘤的临床病理参数之间存在相关性。总之，相当数量但比例较低的结直肠癌EMR2呈阳性，表明该分子和CD97在这些肿瘤中发挥着不同的作用。
