Levitsky Victor G, Katokhin Alexey V
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
In Silico Biol. 2003;3(1-2):81-7. Epub 2003 Feb 27.
A new approach to recognizing promoter regions of eukaryotic genes is proposed and illustrated by an example of Drosophila melanogaster. The essence of its novelty is in realizing the genetic algorithm to search for optimal partition of a promoter region into local nonoverlapping fragments and selection of the most significant dinucleotide frequencies for the fragments obtained. The method developed was applied to recognizing TATA-containing (TATA+) and DPE-containing (DPE+) promoters of Drosophila melanogaster genes. The program for promoter recognition is included into the GeneExpress system, section RegScan (http://wwwmgs.bionet.nsc.ru/mgs/programs/proga/).
本文提出了一种识别真核基因启动子区域的新方法，并以黑腹果蝇为例进行了说明。其新颖之处在于实现了一种遗传算法，用于搜索启动子区域到局部非重叠片段的最优划分，并为所得片段选择最显著的二核苷酸频率。所开发的方法被应用于识别黑腹果蝇基因中含TATA（TATA+）和含DPE（DPE+）的启动子。启动子识别程序包含在GeneExpress系统的RegScan部分（http://wwwmgs.bionet.nsc.ru/mgs/programs/proga/）。
