Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität, Giessen, Germany.
PLoS One. 2010 Jun 17;5(6):e11164. doi: 10.1371/journal.pone.0011164.
Analysis of the complete genomes from the multicellular myxobacteria Myxococcus xanthus and Sorangium cellulosum identified the highest number of eukaryotic-like protein kinases (ELKs) compared to all other genomes analyzed. High numbers of protein phosphatases (PPs) could therefore be anticipated, as reversible protein phosphorylation is a major regulation mechanism of fundamental biological processes.
Here we report an intensive analysis of the phosphatomes of M. xanthus and S. cellulosum in which we constructed phylogenetic trees to position these sequences relative to PPs from other prokaryotic organisms.
PREDOMINANT OBSERVATIONS WERE: (i) M. xanthus and S. cellulosum possess predominantly Ser/Thr PPs; (ii) S. cellulosum encodes the highest number of PP2c-type phosphatases so far reported for a prokaryotic organism; (iii) in contrast to M. xanthus only S. cellulosum encodes high numbers of SpoIIE-like PPs; (iv) there is a significant lack of synteny among M. xanthus and S. cellulosum, and (v) the degree of co-organization between kinase and phosphatase genes is extremely low in these myxobacterial genomes.
We conclude that there has been a greater expansion of ELKs than PPs in multicellular myxobacteria.
与所有分析过的基因组相比，多细胞黏细菌粘球菌和纤维弧菌完整基因组中的真核样蛋白激酶 (ELK) 数量最多。因此，可以预期存在大量的蛋白磷酸酶 (PP)，因为可逆蛋白磷酸化是基本生物过程的主要调控机制。
在这里，我们对粘球菌和纤维弧菌的磷酸组进行了深入分析，构建了系统发育树，将这些序列相对于其他原核生物中的 PP 进行定位。
主要观察结果为：(i) 粘球菌和纤维弧菌主要含有 Ser/Thr PP；(ii) 纤维弧菌编码的 PP2c 型磷酸酶数量是迄今为止报道的原核生物中最多的；(iii) 与粘球菌不同，只有纤维弧菌编码大量的 SpoIIE 样 PP；(iv) 粘球菌和纤维弧菌之间的同线性程度低；(v) 在这些黏细菌基因组中，激酶和磷酸酶基因的组织程度极低。
我们得出结论，在多细胞黏细菌中，ELK 的扩张程度大于 PP。
