Dai Wei, Wang Ning, Wang Wenhui, Ye Xianfeng, Cui Zhongli, Wang Jieling, Yao Dandan, Dong Yuanhua, Wang Hui
CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
University of Chinese Academy of Sciences, Beijing 100000, China.
Microorganisms. 2021 Oct 21;9(11):2193. doi: 10.3390/microorganisms9112193.
Myxobacteria are unique predatory microorganisms with a distinctive social lifestyle. These taxa play key roles in the microbial food webs in different ecosystems and regulate the community structures of soil microbial communities. Compared with conditions under conventional management, myxobacteria abundance increases in the organic soil, which could be related to the presence of abundant myxobacteria in the applied compost manure during organic conditions. In the present study,16S rRNA genes sequencing technology was used to investigate the community profile and drivers of predatory myxobacteria in four common compost manures. According to the results, there was a significant difference in predatory myxobacteria community structure among different compost manure treatments ( < 0.05). The alpha-diversity indices of myxobacteria community under swine manure compost were the lowest (Observed OTU richness = 13.25, Chao1 = 14.83, Shannon = 0.61), and those under wormcast were the highest (Observed OTU richness = 30.25, Chao1 = 31.65, Shannon = 2.62). Bacterial community diversity and Mg and Ca concentrations were the major factors influencing the myxobacteria community under different compost manure treatments. In addition, organic carbon, pH, and total nitrogen influenced the community profile of myxobacteria in compost manure. The interaction between myxobacteria and specific bacterial taxa (Micrococcales) in compost manure may explain the influence of bacteria on myxobacteria community structure. Further investigations on the in-situ community profile of predatory myxobacteria and the key microorganism influencing their community would advance our understanding of the community profile and functions of predatory microorganisms in the microbial world.
粘细菌是独特的捕食性微生物，具有独特的社会生活方式。这些分类群在不同生态系统的微生物食物网中发挥关键作用，并调节土壤微生物群落的结构。与传统管理条件相比，有机土壤中粘细菌的丰度增加，这可能与有机条件下施用的堆肥中存在大量粘细菌有关。在本研究中，利用16S rRNA基因测序技术研究了四种常见堆肥中捕食性粘细菌的群落特征及其驱动因素。结果表明，不同堆肥处理间捕食性粘细菌群落结构存在显著差异（<0.05）。猪粪堆肥下粘细菌群落的α多样性指数最低（观察到的OTU丰富度=13.25，Chao1=14.83，香农指数=0.61），而蚯蚓粪下的α多样性指数最高（观察到的OTU丰富度=30.25，Chao1=31.65，香农指数=2.62）。细菌群落多样性以及镁和钙的浓度是影响不同堆肥处理下粘细菌群落的主要因素。此外，有机碳、pH值和总氮影响堆肥中粘细菌的群落特征。堆肥中粘细菌与特定细菌分类群（微球菌目）之间的相互作用可能解释了细菌对粘细菌群落结构的影响。对捕食性粘细菌原位群落特征及其群落关键微生物的进一步研究，将有助于我们深入了解微生物世界中捕食性微生物的群落特征和功能。
