Mailer Colin, Robinson Bruce H, Williams Benjamin B, Halpern Howard J
Center for EPR Imaging In Vivo Physiology, Department of Radiation and Cellular Oncology, University of Chicago, Illinois 60637, USA.
Magn Reson Med. 2003 Jun;49(6):1175-80. doi: 10.1002/mrm.10474.
A highly accurate line-width simulation computer program is used that can account for both high amplitude and frequency of the Zeeman modulation in an electron paramagnetic resonance (EPR) experiment. This allows for the overmodulation of EPR lines to increase signal-to-noise ratio (SNR) in EPR spectra and spectroscopic images, without any sacrifice in the determination of the intrinsic line width (1/gamma. T(2e)). The technique was applied to continuous-wave EPR spectroscopic images of a narrow, single-line trityl spin probe wherein a full EPR spectrum was extracted from each 3D spatial voxel. Typical improvements are a three- to fivefold increase in SNR in the high-gradient projections in the image and a reduction in the standard deviation (SD), by a factor of 3, of the line widths in the low-gradient domain. This method is a general one that is also applicable to the analysis of conventional (14)N or (15)N nitroxide spin probes.
使用了一种高精度线宽模拟计算机程序，该程序能够考虑电子顺磁共振（EPR）实验中塞曼调制的高幅度和频率。这使得EPR谱线能够过调制，以提高EPR谱和光谱图像中的信噪比（SNR），而在确定固有线宽（1/γ·T(2e)）时不会有任何损失。该技术应用于窄单线三苯甲基自旋探针的连续波EPR光谱图像，其中从每个3D空间体素中提取完整的EPR谱。典型的改进是图像中高梯度投影的信噪比提高三到五倍，低梯度域中线宽的标准偏差（SD）降低三倍。该方法是一种通用方法，也适用于传统（14）N或（15）N氮氧化物自旋探针的分析。
