Janković-Hladni M, Ivanović J, Spasić M B., Blagojević D, Perić-Mataruga V
Institute for Biological Research 'Sinisa Stanković', 29.Novembra 142, 11000, Belgrade, Yugoslavia
J Insect Physiol. 1997 Feb 19;43(1):101-106. doi: 10.1016/s0022-1910(96)00018-2.
THE RESPONSES OF GYPSY MOTH LARVAE ORIGINATING FROM TWO POPULATIONS (OAK FOREST, LOCUST FOREST) TO FAVORABLE (OAK) AND UNFAVORABLE (LOCUST) HOST PLANTS WERE MONITORED AT THE LEVEL OF MIDGUT ANTIOXIDATIVE DEFENCE: the activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase like ('GSH-Px like') and glutathione content (GSH). Short-term change of the diet (3 days) to locust leaves of the 5th instar larvae (oak population) provoked an increase in GST and 'GSH-Px like' activities as well as in the amount of GSH. On the contrary, transferring the gypsy moth larvae (locust population) to oak leaves was followed by a decrease in GST, 'GSH-Px like' activities, and in the amount of GSH. Feeding gypsy moth larvae from hatching on an unfavorable host plant such as locust, led to increases in GST and SOD activities and GSH content, as well as to a decrease in CAT activity in all instars studied (4th, 5th, 6th). The locust leaf diet caused changes in other components of antioxidative defence dependent on larval instar and population origin, a feature which could be ascribed to trophic adaptation of the gypsy moth to an unfavorable host plant.
在中肠抗氧化防御水平上监测了来自两个种群（橡树林、刺槐林）的舞毒蛾幼虫对适宜宿主植物（橡树）和不适宜宿主植物（刺槐）的反应：超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽 - S - 转移酶（GST）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶样（“GSH - Px样”）的活性以及谷胱甘肽含量（GSH）。将五龄幼虫（橡树种群）的食物短期（3天）更换为刺槐叶，会导致GST和“GSH - Px样”活性以及GSH含量增加。相反，将舞毒蛾幼虫（刺槐种群）转移到橡树叶上后，GST、“GSH - Px样”活性以及GSH含量会降低。从孵化开始就以不适宜的宿主植物如刺槐喂养舞毒蛾幼虫，会导致所研究的所有龄期（第四、五、六龄）的GST和SOD活性以及GSH含量增加，同时CAT活性降低。刺槐叶食物会导致抗氧化防御的其他成分发生变化，这种变化取决于幼虫龄期和种群来源，这一特征可归因于舞毒蛾对不适宜宿主植物的营养适应。
