Central Tasar Research and Training Institute, Piska Nagri, Ranchi, Jharkhand, India.
J Insect Sci. 2013;13:123. doi: 10.1673/031.013.12301.
In the present study, the total hydroperoxides, catalase, glutathione-s-transferase, and ascorbic acid contents were determined in different developmental stages of the non-diapause and the diapause generation of the tropical tasar silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae). The results showed stage-specific significantly higher levels of total hydroperoxides, catalase, and ascorbic acid contents in the non-diapause as compared to the diapause generation (p < 0.05). However, a significantly enhanced level of glutathione-S-transferase activity was observed in mature 5th instar larvae of the diapause generation (p < 0.05). In the case of pupae, significantly higher levels of total hydroperoxides, catalase, and glutathione-s-transferase activity were observed in the non-diapause generation (p < 0.05). These results could be the effect of intensive metabolic transformation that takes place in tissues of the non-diapause generation and causes increased production of reactive oxygen species, such as hydroperoxides. The results suggest that antioxidants play an important role in protecting cells against reactive oxygen species.
在本研究中，测定了非滞育和滞育世代的热带马桑蚕（鳞翅目：Saturniidae）不同发育阶段的总过氧化物、过氧化氢酶、谷胱甘肽-S-转移酶和抗坏血酸含量。结果表明，与滞育世代相比，非滞育世代的总过氧化物、过氧化氢酶和抗坏血酸含量具有明显的阶段特异性升高（p<0.05）。然而，滞育世代的成熟 5 龄幼虫中观察到谷胱甘肽-S-转移酶活性显著增强（p<0.05）。在蛹期，非滞育世代的总过氧化物、过氧化氢酶和谷胱甘肽-S-转移酶活性明显升高（p<0.05）。这些结果可能是由于非滞育世代组织中发生的剧烈代谢转化导致活性氧（如过氧化物）产生增加的结果。结果表明，抗氧化剂在保护细胞免受活性氧的侵害方面发挥着重要作用。
