Barlogie B, Drewinko B, Benjamin R S, Freireich E J
Cancer Res. 1976 Jul;36(7 PT 1):2540-4.
The lysosomotropic agent adriamycin-DNA complex was analyzed for its effect on cell cycle progression of human lymphoid cells in culture by means of pulse cytophotometry. Complexing to DNA slightly reduced the perturbation effects previously reported for adriamycin alone. The major kinetic response was a G2 block, the magnitude and duration of which was dependent on drug concentration and duration of treatment. When high drug concentrations were maintained for a prolonged period of time, an additional, completely reversible block in G1 phase or at the G1-S boundary was observed, accounting for two-step G2 accumulation curves. Cell age markedly influenced the magnitude of G2 accumulation in that treatment of cells in S and early G2 phase was most effective.
采用脉冲细胞光度法分析了溶酶体亲和剂阿霉素-DNA复合物对培养的人淋巴细胞细胞周期进程的影响。与DNA结合略微降低了先前单独报道的阿霉素的干扰作用。主要的动力学反应是G2期阻滞，其程度和持续时间取决于药物浓度和处理时间。当长时间维持高药物浓度时，在G1期或G1-S边界观察到另一个完全可逆的阻滞，这解释了两步G2期积累曲线。细胞年龄显著影响G2期积累的程度，即对处于S期和G2早期的细胞进行处理最为有效。
