[不同配伍的半夏泻心汤中某些化学成分的变化]
[The changes of some chemical components in banxiaxiexintang decoction of different combinations].
作者信息
Duan Tian-xuan, Ma Chang-hua, Chen Tao, Lun Li-hui, Okuma Ryoko, Li Yuhang
机构信息
College of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2002 May;27(5):363-5.
OBJECTIVE
The contents of bererine, palmatine and glycyrrhizin acid in Banxiaxiexintang decoction of different combinations were determined by PR-HPLC.
METHOD
A Shim-pack CLC-ODS column was used with a mobile phase of CH3CN-H2O (31:69; 0.005 moL.L-1 -pentanesulfonic acid sodium salt, H3PO4: pH 3.0) for bererine andpalmatine, which were detected at the wavelength of 275 nm. A YWG-C18 column was used with a mobile phase of CH3OH-H2O-HAc(62:37:1) for glycyrrhizin acid which was detected at the wavelength of 260 nm.
RESULT
Each herbs' combination influences the contents of the 3 components.
CONCLUSION
The experiment is an attempt to study the comical foundation of traditional Chinese prescription.
目的
采用PR-HPLC法测定不同配伍的半夏泻心汤中黄连素、巴马汀和甘草酸的含量。
方法
采用Shim-pack CLC-ODS柱，以CH3CN-H2O（31:69；0.005 moL.L-1戊烷磺酸钠盐，H3PO4：pH 3.0）为流动相测定黄连素和巴马汀，检测波长为275 nm。采用YWG-C18柱，以CH3OH-H2O-HAc（62:37:1）为流动相测定甘草酸，检测波长为260 nm。
结果
各药味配伍对3种成分的含量有影响。
结论
本实验是研究中药复方配伍规律的一次尝试。
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