Bailey D N, Jatlow P I
Clin Chem. 1976 Jun;22(6):777-81.
We describe a gas-chromatographic procedure for the simultaneous determination of amitriptyline and its active metabolite, nortriptyline, in therapeutic concentrations in human plasma, with use of a nitrogen detector. Both drugs are extracted at pH 10.5 into hexane/isoamyl alcohol, back-extracted into dilute HCl, and re-extracted into hexane/isoamyl alcohol after alkalinization of the HCl. The solvent is evaporated and the residue gas-chromatographed. Protriptyline is used as the internal standard. As little as 5 mug of amitriptyline or nortriptyline can be detected per liter of plasma. The coefficients of variation, for a concentration of 200 mug/liter, are 4.6% and 4.3% within-day and 8.6% and 3.4% day-to-day for amitriptyline and nortriptyline, respectively. The procedure was applied to patients receiving therapeutic doses of both drugs and also to patients who had taken overdoses of amitriptyline.
我们描述了一种气相色谱法，用于同时测定人血浆中治疗浓度的阿米替林及其活性代谢物去甲替林，使用氮检测器。两种药物在pH 10.5时被萃取到己烷/异戊醇中，反萃取到稀盐酸中，盐酸碱化后再萃取到己烷/异戊醇中。溶剂蒸发后，残渣进行气相色谱分析。普罗替林用作内标。每升血浆中可检测到低至5微克的阿米替林或去甲替林。对于浓度为200微克/升的情况，阿米替林和去甲替林的日内变异系数分别为4.6%和4.3%，日间变异系数分别为8.6%和3.4%。该方法应用于接受两种药物治疗剂量的患者以及过量服用阿米替林的患者。
