Weiss R, Freudenschuss M, Krska R, Mizaikoff B
Vienna University of Technology, Institute for Chemical Technologies and Analytics, Getreidemarkt 9/151, A-1060 Vienna, Austria.
Food Addit Contam. 2003 Apr;20(4):386-95. doi: 10.1080/0265203031000065827.
Selective polymeric phases intended for future use in separation/extraction of deoxynivalenol and zearalenone from beverages have been prepared. Using crystalline deoxynivalenol, zearalenone and quercetin, molecularly imprinted polymers were obtained by a non-covalent imprinting approach via a photo-initiated addition polymerization. Prepared polymers were based on 4-vinylpyridine, methacrylic acid or 2-trifluoromethylacrylic acid as the functional monomer and on ethyleneglycol dimethacrylate, trimethyltrimethacrylate or divinylbenzene as the cross-linking monomer. Selectivity of the generated molecularly imprinted polymers has been investigated by application of the prepared molecularly imprinted polymers as stationary phases in high-pressure liquid chromatography experiments. The retention and elution behaviours of the template compounds and structurally related substances were determined and compared. The results promise future application of molecularly imprinted polymers as alternative selective matrices for clean-up and enrichment of deoxynivalenol and zearalenone.
已制备出用于未来从饮料中分离/提取脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的选择性聚合物相。使用结晶性脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮和槲皮素，通过光引发加成聚合的非共价印迹方法获得了分子印迹聚合物。制备的聚合物以4-乙烯基吡啶、甲基丙烯酸或2-三氟甲基丙烯酸作为功能单体，以乙二醇二甲基丙烯酸酯、三甲基三甲基丙烯酸酯或二乙烯基苯作为交联单体。通过将制备的分子印迹聚合物用作高压液相色谱实验中的固定相，研究了所生成的分子印迹聚合物的选择性。测定并比较了模板化合物和结构相关物质的保留和洗脱行为。结果表明分子印迹聚合物有望作为替代选择性基质用于脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的净化和富集。
