高效液相色谱法测定补骨脂中两种黄酮类化合物
[HPLC determination of two flavonoid compounds in Psoralea corylifolia].
作者信息
Lin Rui-min, Wang Da-wei, Xiong Zhi-li, Xu Yong, Li Fa-mei
机构信息
Department of Chemistry, Anshan Teacher's College, Anshan 114005, Liaoning, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2002 Sep;27(9):669-71.
OBJECTIVE
To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method.
METHOD
Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol.L-1 ammonium acetate buffer pH 4.0 (67:33), and the UV detection wavelength was 240 nm.
RESULT
Simultaneous determination of bavachin and corylin was achieved. The linear range was 1.25-20 micrograms.mL-1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively.
CONCLUSION
This is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.
目的
采用高效液相色谱法同时测定补骨脂中的两种黄酮类化合物。
方法
以本实验室从补骨脂中分离纯化得到的补骨脂二氢黄酮甲醚和补骨脂宁为对照品。采用Techsphere ODS柱进行高效液相色谱分离，流动相为甲醇和20 mmol·L⁻¹pH 4.0的醋酸铵缓冲液（67:33）混合溶液，紫外检测波长为240 nm。
结果
实现了补骨脂二氢黄酮甲醚和补骨脂宁的同时测定。补骨脂二氢黄酮甲醚和补骨脂宁的线性范围均为1.25 - 20μg·mL⁻¹。补骨脂二氢黄酮甲醚和补骨脂宁的平均回收率分别为94.9%和96.2%，相对标准偏差分别为3.1%和3.6%。
结论
这是首次同时测定补骨脂中补骨脂二氢黄酮甲醚和补骨脂宁的报道，准确度和重现性良好。
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