[高效液相色谱-质谱联用技术鉴定补骨脂果实中的化合物]

[Identification of compounds in fruits of Psoralea corylifolia by HPLC-MS].

作者信息

Liu Ya'nan, Wang Yuefei, Han Lifeng, Pan Guixiang, Wang Hong

机构信息

Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique Traditional Chinese Medicine, Research Centre of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2898-902.

PMID:20209955

Abstract

OBJECTIVE

To identify the compounds in fruits of Psoralea corylifolia by high performance liquid chromatography-mass spectrometry.

METHOD

The chromatographic separation was carried out at 30 degrees C on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) eluted in the gradient program. The mobile phase consisted of acetonitrile (A) and water containing 0.05% formic acid (B). The detection wavelength was at 246 nm and the on-line UV spectra was recorded in the range of 190-400 nm. The mass spectra were obtained by Finnigan LCQ Deca XP(plus) ion trap mass spectrometer in the positive ion mode with ion spray voltage 4.5 kV, sheath gas (N2), 60 arbitrary units, auxiliary gas (N2), 20 arbitrary units; capillary temperature, 350 degrees C, capillary voltage 19 V, tube lens offset voltage 25 V, mass range recorded, m/z 90-800.

RESULT

Two coumarin glycosides, three coumarins, eight flavonoids and one meroterpene were identified by HPLC-MS method.

CONCLUSION

The method is simple and rapid for the identification and quality control for the fruits of P. corylifolia.

摘要

目的

采用高效液相色谱 - 质谱联用技术鉴定补骨脂果实中的化合物。

方法

在30℃下，于Diamonsil C18柱（4.6 mm×250 mm，5μm）上进行色谱分离，采用梯度洗脱程序。流动相由乙腈（A）和含0.05%甲酸的水（B）组成。检测波长为246 nm，在线紫外光谱在190 - 400 nm范围内记录。质谱通过Finnigan LCQ Deca XP（plus）离子阱质谱仪在正离子模式下获得，离子喷雾电压4.5 kV，鞘气（N2）60任意单位，辅助气（N2）20任意单位；毛细管温度350℃，毛细管电压19 V，管透镜偏移电压25 V，记录的质量范围为m/z 90 - 800。