Heegaard S, Spang-Thomsen M, Prause J U
Eye Pathology Institute, University of Copenhagen, Copenhagen, Denmark.
Melanoma Res. 2003 Jun;13(3):247-51. doi: 10.1097/00008390-200306000-00004.
The purpose of this study was to develop a suitable animal model for the investigation of the pathogenesis and therapy of uveal malignant melanoma. Eight choroidal malignant melanomas from eight patients were transplanted into nude mice in an attempt to establish a serially transplantable tumour model. Tumour tissue blocks (2 x 2 x 2 mm) from enucleated eyes with choroidal malignant melanoma were transplanted subcutaneously into the flanks of nude mice. The growing tumours were measured and serially transplanted. The tumour samples were investigated by histology, immunohistochemistry and electron microscopy. Only one of the eight transplanted primary tumours (13%) was established as a xenograft in nude mice. Furthermore, the take rate of the transplantable tumour was low (13%). The growth of the tumour fitted a Gompertz function, and the calculated tumour volume doubling time was 54 days. The transplanted tumour cells were epithelioid and slightly larger than the primary tumour cells and had prominent nucleoli. However, the transplanted tumour retained a morphological appearance similar to that of the primary tumour. Immunohistochemical examinations demonstrated that the cells preserved the characteristic properties of malignant melanoma. However, the transplanted cells demonstrated vimentin reactivity, whereas the primary tumour cells were negative for vimentin. It can be concluded that a new experimental model of malignant uveal melanoma with tumours that were easy to observe and access was established in nude mice.
本研究的目的是建立一种合适的动物模型，用于研究葡萄膜恶性黑色素瘤的发病机制和治疗方法。将8例患者的8个脉络膜恶性黑色素瘤移植到裸鼠体内，试图建立一种可连续传代移植的肿瘤模型。将摘除的患有脉络膜恶性黑色素瘤的眼球中的肿瘤组织块（2×2×2毫米）皮下移植到裸鼠的侧腹。对生长中的肿瘤进行测量并连续传代移植。通过组织学、免疫组织化学和电子显微镜对肿瘤样本进行研究。8个移植的原发性肿瘤中只有1个（13%）在裸鼠体内成功建立异种移植模型。此外，可移植肿瘤的接种率较低（13%）。肿瘤的生长符合Gompertz函数，计算得出的肿瘤体积倍增时间为54天。移植的肿瘤细胞呈上皮样，比原发性肿瘤细胞略大，核仁明显。然而，移植的肿瘤在形态学上与原发性肿瘤相似。免疫组织化学检查表明，这些细胞保留了恶性黑色素瘤的特征性特性。然而，移植的细胞显示波形蛋白反应阳性，而原发性肿瘤细胞波形蛋白呈阴性。可以得出结论，在裸鼠体内建立了一种新的易于观察和取材的恶性葡萄膜黑色素瘤实验模型。
