Jager Martine J, Magner J Antonio Bermudez, Ksander Bruce R, Dubovy Sander R
Department of Ophthalmology, Leiden University Medical Center, Leiden, The Netherlands (Dr Jager); Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts (Dr Jager, Dr Ksander); Florida Lions Ocular Pathology Laboratory, Bascom Palmer Eye Institute, University of Miami, Miami, Florida (Dr Magner, Dr Dubovy); and Instituto de Oftalmologia Conde de Valenciana, Mexico City, Mexico (Dr Magner).
Trans Am Ophthalmol Soc. 2016 Aug;114:T5.
To determine whether some of the most often used uveal melanoma cell lines resemble their original tumor.
Analysis of the literature, patient charts, histopathology, mutations, chromosome status, HLA type, and expression of melanocyte markers on cell lines and their primary tumors. We examined five cell lines and the primary tumors from which they were derived.
Four of the five examined primary tumors were unusual: one occupied the orbit, two were recurrences after prior irradiation, and one developed in an eye with a nevus of Ota. One cell line did not contain the GNA11 mutation, but it was present in the primary tumor. Three of the primary tumors had monosomy 3 (two of these lacked BAP1 expression); however, all five cell lines showed disomy 3 and BAP1 expression. All of the cell lines had gain of 8q. Two cell lines lacked expression of melanocyte markers, although these were present in the corresponding primary tumor.
All cell lines could be traced back to their original uveal melanoma. Four of the five primary tumors were unusual. Cell lines often differed from their primary tumor in chromosome status and melanocyte markers. However, their specific chromosome aberrations and capacity to continue proliferation characterize them as uveal melanoma cell lines.
确定一些最常用的葡萄膜黑色素瘤细胞系是否与其原发肿瘤相似。
对文献、患者病历、组织病理学、突变、染色体状态、HLA类型以及细胞系及其原发肿瘤上黑素细胞标志物的表达进行分析。我们检查了五个细胞系及其来源的原发肿瘤。
所检查的五个原发肿瘤中有四个情况特殊：一个占据眼眶，两个是先前放疗后的复发肿瘤，一个发生于患有太田痣的眼睛。一个细胞系不含有GNA11突变，但该突变存在于原发肿瘤中。三个原发肿瘤有3号染色体单体性（其中两个缺乏BAP1表达）；然而，所有五个细胞系均显示3号染色体二体性且有BAP1表达。所有细胞系均有8q获得。两个细胞系缺乏黑素细胞标志物的表达，尽管这些标志物在相应的原发肿瘤中存在。
所有细胞系均可追溯至其原发葡萄膜黑色素瘤。五个原发肿瘤中有四个情况特殊。细胞系在染色体状态和黑素细胞标志物方面常常与其原发肿瘤不同。然而，它们特定的染色体畸变和持续增殖能力使其具有葡萄膜黑色素瘤细胞系的特征。
