Quasie O, Martey O N K, Nyarko A K, Gbewonyo W S K, Okine L K N
Centre for Scientific Research into Plant Medicine, Mampong-Akuapem, Ghana.
Afr J Tradit Complement Altern Med. 2010 Apr 3;7(3):241-52. doi: 10.4314/ajtcam.v7i3.54783.
Mondia whitei root was evaluated to validate its anecdotal use and determine its possible mode of action in the management of erectile dysfunction. Rabbits were administered with daily oral doses of 100-400 mg kg(-1) crude ethanolic extract of M. whitei and sildenafil (50 mg kg(-1)) as positive control for 6 weeks. Cavernosal tissue NOS activity and levels of NO and cGMP, and NOS and PDE protein expressions were investigated. The effect of the crude extract, chloroform and petroleum ether fractions in vitro on cavernosal tissue NOS activity and levels of NO and cGMP at 0.01 and 0.10 mg g(-1) tissue were also investigated. Results indicate that the crude extract increased NOS activity by 7% at 200 mg kg(-1) with corresponding increases in NO (88%) and cGMP (480%) levels. No significant changes in these measurements were observed with the 100 and 400 mg kg(-1) doses whilst sildenafil slightly reduced them (15.9-37.5%). NOS and PDE protein expressions in test animals were not different from controls. Pre-incubation of cavernosal tissue in vitro with the crude extract of M. whitei and its chloroform fraction markedly increased NOS activity (26-132%) and levels of NO (25%) and cGMP (50-400%) at 0.01 mg g(-1) tissue but these were reduced to near control levels when their concentrations were increased to 0.10 mg g(-1) tissue whilst the petroleum ether fraction had no effect. These findings suggest that M. whitei may influence erectile function through activation/stimulation of NOS with corresponding increases in tissue NO and cGMP levels and that certain chemical constituents present in the chloroform fraction may be responsible for biological activity.
对白叶蒙迪亚根进行评估，以验证其传闻中的用途，并确定其在治疗勃起功能障碍中的可能作用方式。给兔子每日口服100 - 400 mg kg(-1)的白叶蒙迪亚粗乙醇提取物，以50 mg kg(-1)的西地那非作为阳性对照，持续6周。研究了海绵体组织一氧化氮合酶（NOS）活性、一氧化氮（NO）和环磷酸鸟苷（cGMP）水平，以及NOS和磷酸二酯酶（PDE）蛋白表达。还研究了粗提取物、氯仿和石油醚馏分在体外对海绵体组织NOS活性以及在0.01和0.10 mg g(-1)组织时NO和cGMP水平的影响。结果表明，粗提取物在200 mg kg(-1)时使NOS活性增加了7%，同时NO（88%）和cGMP（480%）水平相应增加。100和400 mg kg(-1)剂量下这些测量值无显著变化，而西地那非使其略有降低（15.9 - 37.5%）。试验动物中的NOS和PDE蛋白表达与对照组无差异。体外将海绵体组织与白叶蒙迪亚粗提取物及其氯仿馏分预孵育，在0.01 mg g(-1)组织时显著增加了NOS活性（26 - 132%）以及NO（25%）和cGMP（50 - 400%）水平，但当它们的浓度增加到0.10 mg g(-1)组织时，这些水平降至接近对照水平，而石油醚馏分没有影响。这些发现表明，白叶蒙迪亚可能通过激活/刺激NOS来影响勃起功能，相应增加组织中NO和cGMP水平，并且氯仿馏分中存在的某些化学成分可能是生物活性的原因。
