Martin W John
Center for Complex Infectious Diseases, Rosemead, CA 91770, USA.
Exp Mol Pathol. 2003 Jun;74(3):210-23. doi: 10.1016/s0014-4800(03)00037-6.
The cytopathic effect (CPE) induced in human MRC-5 fibroblasts by blood mononuclear cells of patients infected with stealth-adapted viruses is characterized by the formation of clusters of foamy vacuolated cells that commonly become heavily pigmented. The pigmented material coalesces into discrete structures of varying shapes and sizes, including solid particles, flat ribbons, and long, thin threads. Pigmented material can also be observed in long-term culture supernatants, sometimes accompanied by solid and needle-shaped lipid-like crystals. Accumulation of the pigmented material correlates with lessening of the CPE and outgrowth of normal appearing cells from cell clusters. The CPE can be rapidly reactivated by replacing the culture supernatant with fresh culture medium. Conversely, reactivation can be partially prevented by the addition of particulate pigmented material to fresh culture medium. The pigmented material displays striking luminescence and autofluorescence. Occasional particles are ferromagnetic. Varying percentages of different minerals are identifiable in the particles using energy dispersive X-ray (EDX) analysis. Metabolic studies indicate the particles can have reducing (electron donating) capacity and can liberate gas. These findings, along with the extended survival of viable cells in unfed virus-infected cultures, suggest that the pigments are a potential source of cellular energy. The presence of light and magnetic-sensitive alternative cellular energy (ACE) pigments has opened new approaches for the potential therapy of stealth virus-infected patients.
感染了隐形适应病毒的患者血液单核细胞在人MRC - 5成纤维细胞中诱导产生的细胞病变效应（CPE）的特征是形成泡沫状空泡化细胞簇，这些细胞通常会大量色素沉着。色素沉着物质聚合成形状和大小各异的离散结构，包括固体颗粒、扁平条带和细长的线状物。在长期培养上清液中也可观察到色素沉着物质，有时还伴有固体和针状脂质样晶体。色素沉着物质的积累与CPE的减轻以及细胞簇中正常外观细胞的生长相关。通过用新鲜培养基替换培养上清液，CPE可迅速重新激活。相反，向新鲜培养基中添加颗粒状色素沉着物质可部分阻止重新激活。色素沉着物质显示出显著的发光和自发荧光。偶尔的颗粒具有铁磁性。使用能量色散X射线（EDX）分析可在颗粒中鉴定出不同矿物质的不同百分比。代谢研究表明，这些颗粒具有还原（电子供体）能力并能释放气体。这些发现，连同未喂食病毒感染培养物中活细胞的延长存活时间，表明这些色素是细胞能量的潜在来源。对光和磁敏感的替代细胞能量（ACE）色素的存在为隐形病毒感染患者的潜在治疗开辟了新途径。
