儿童眼表真菌的分子分析
Molecular analysis of the pediatric ocular surface for fungi.
作者信息
Wu Tzu, Mitchell Bradley, Carothers Thomas, Coats David, Brady-McCreery Kathryn, Paysse Evelyn, Wilhelmus Kirk
机构信息
Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA.
出版信息
Curr Eye Res. 2003 Jan;26(1):33-6. doi: 10.1076/ceyr.26.1.33.14253.
PURPOSE
To analyze the conjunctival flora of individuals 21 years of age or less for fungi using polymerase chain reaction (PCR) methodology.
METHODS
Before povidone-iodine antisepsis, eye-swab specimens were collected from adolescent corneal donors preceding corneal excision and from children during preparation for strabismus surgery. Nucleic acid was extracted from the specimens and analyzed by PCR using primers designed for the detection of broad-spectrum fungal DNA and of Candida albicans-specific DNA.
RESULTS
Twelve (38%) of 32 eye donor surfaces and 7 (23%) of 30 patient samples were positive for fungal DNA (P = 0.1). C. albicans DNA was detected in 6 (19%) of the decedents' eyes but from none of the surgical patients (P = 0.04).
CONCLUSIONS
Fungi were present on the normal ocular surface of children and adolescents. C. albicans was more likely to be found postmortem than pre-surgically.
目的
使用聚合酶链反应（PCR）方法分析21岁及以下个体结膜菌群中的真菌。
方法
在聚维酮碘消毒前，从青少年角膜供体角膜切除术前的眼拭子标本以及斜视手术准备期间儿童的眼拭子标本中采集样本。从标本中提取核酸，并使用为检测广谱真菌DNA和白色念珠菌特异性DNA设计的引物通过PCR进行分析。
结果
32个眼供体表面中的12个（38%）和30个患者样本中的7个（23%）真菌DNA呈阳性（P = 0.1）。在6名（19%）死者的眼中检测到白色念珠菌DNA，但在手术患者中均未检测到（P = 0.04）。
结论
真菌存在于儿童和青少年的正常眼表。白色念珠菌在死后比术前更易被发现。
Zotero 插件