Kim K K, Hwang K Y, Jeon H S, Kim S, Sweet R M, Yang C H, Suh S W
Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.
J Mol Biol. 1992 Oct 20;227(4):1258-62. doi: 10.1016/0022-2836(92)90537-t.
Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1.0 mm x 0.5 mm x 0.3 mm. The crystals belong to the monoclinic space group P2(1), with unit cell parameters a = 84.91 A, b = 47.33 A, c = 86.00 A, and beta = 116.09 degrees. And they diffract to about 1.6 A upon exposure to synchroton X-rays. X-ray data have been collected to 2.2 A Bragg spacing from a native crystal.
洋葱伯克霍尔德氏菌脂肪酶的大晶体已在室温下从含有2-甲基-2,4-戊二醇和柠檬酸钠的溶液中生长出来。它们在两周内生长到典型尺寸为1.0毫米×0.5毫米×0.3毫米。这些晶体属于单斜空间群P2(1)，晶胞参数为a = 84.91埃，b = 47.33埃，c = 86.00埃，β = 116.09度。并且在同步加速器X射线照射下，它们的衍射分辨率约为1.6埃。已从天然晶体收集到布拉格间距为2.2埃的X射线数据。
