Dymond J B, Kalmus G W
Department of Biology, East Carolina University, Greenville, N.C. 27858.
Prostaglandins. 1992 Aug;44(2):129-34. doi: 10.1016/0090-6980(92)90074-4.
Prostaglandins (PGs) have been shown to cytoprotect various tissue types against the toxic effects of many chemicals. The mechanism of this protection is poorly understood, but the involvement of cAMP is often implied. Only one previous study examined nervous tissue and PG protection. The present study was designed to determine if PGE2 affords cytoprotection to a more specific nervous tissue (embryonic neural retina) from the toxicity of actinomycin C (AMC) using a trypan blue exclusion assay. The lowest concentration of PGE2 (2 x 10(-5)M) had no effect, but as the concentration increased (3 x 10(-5)M and 5 x 10(-5)M), PGE2 did afford protection against AMC in a dose dependent fashion. Theophylline treated cells were not protected, suggesting that cAMP may not be the primary mechanism of protection.
前列腺素（PGs）已被证明能对多种组织类型起到细胞保护作用，使其免受许多化学物质的毒性影响。这种保护机制尚不清楚，但常常暗示cAMP参与其中。之前仅有一项研究检测了神经组织和PG保护作用。本研究旨在使用台盼蓝排斥试验确定PGE2是否能为更特定的神经组织（胚胎神经视网膜）提供细胞保护，使其免受放线菌素C（AMC）的毒性影响。最低浓度的PGE2（2×10⁻⁵M）没有效果，但随着浓度增加（3×10⁻⁵M和5×10⁻⁵M），PGE2确实以剂量依赖的方式为AMC提供了保护。用茶碱处理的细胞未受到保护，这表明cAMP可能不是主要的保护机制。
